| Pinus tabulaeformis which is wide distribution,grow fast,well quality, dry conditions barren,distributed14province(areas)in north china,is the most main coniferous forest tree species. Widely used in the pulp and paper, building materials, supporting the our country many paper, wood processing, forest chemical industry development.This paper mainly for domestic earlier established and has entered into the normal period of flowering strong second-generation clone seed orchard, research and reveals the parents grow, the second generation of clone seed orchard genetic diversity, in order for the second generation of pinus tabulaeformis seed orchard construction and of high quality and high yield of genetic management to provide important scientific theory basis. The main research results are as follows:(1) Established the use of improved CTAB method, to tender plant material for pinus tabulaeformis, extraction method of high quality genomic DNA for RAPD.(2) Article118random primers from selected article15(S03, S19, S20back, S46S50S52, S91.S193S243S248S283, S299, S384, S485, S499),118a second generation of pinus tabulaeformis seed orchard clone DNA samples amplification, a total of201bands out amplification, including polymorphisms channel article152, accounting for75.62%of the total number of bands amplification, different primer amplification fragments range number, number of bands expansion at least primer for S19, only article11; The maximum number of bands and expansion of the primer for S193, article16. Each primer amplification a points in11-16, and between the average each primer amplification of a points for13.4, amplified DNA fragments in length from200to2000between bp, shows the second generation of pinus tabulaeformis seed garden between the son of clone abundant genetic diversity and complexity.(3) Second-generation of pinus tabulaeformis seed orchard high genetic diversity. Nei’s genetic diversity index (H) is0.3721, shannon’s information index (â… ) is0.4352, the total polymorphic loci percentage is75.62%.(4) Used groups by UPGMA cluster established the genetic relationship figure. In the genetic similarity coefficient077level up and will be118or four types of pinus tabulaeformis cluster(5) on the whole, experience the first generation breeding and high strength after the selection, the second generation of pinus tabulaeformis breeding population genetic variation basis is not significantly reduce, The level of genetic diversity in the second clonal seed orchard did not decrease with the increasing of artificial selection strength。... |
