Studies On Differential Expressing Proteins In Rice Sterile Lines Infected With Blast

Rice blast is one of the most devastating fungal diseases. Breeding new ricevarieties with blast-resistance ability is an important way to get rice with high yieldand good quality. In this paper, rice sterile lines with blast-resistance ability were employed as materials. The differential expressed proteins in rice varieties, which wereinfected with M. grisea strain “98022B” at four-leaf stage for24hours and72hours,were analyzed by using proteomic methods.1. At the stage of rice infected with blast24hours, we found that RuBisCO largechain precursor in Gunong13and Tiangu; RuBisCO large subunit (precursor) inQuanfeng, Gufeng and the control LTH; cytochrome b6-f complex iron-sulfur subunitin Gunong13and Fuyi; ATP synthetase subunit in Gunong13, Quanfeng and Tiangu,uridylyltransferase-related protein in Tiangu, Gufeng and LTH, fruit protein in Fuyiand Gufeng were all down-regulated. We also found some specific proteins in specificrice varieties: such as elongation factor and ribosomal protein L12homolog in Gunong13, OEC polypeptide and fibrillin-like protein in Quanfeng; ascorbate peroxidate inGufeng, ribulose-5-phosphate isomerase, Clp protease homologue and2-cys peroxiredoxin bas1in LTH were all down-regulated, while OEC polypeptide in LTH andShoot1protein in Quanfeng were up-regulated.2. At the stage of rice infected with blast72hours, RuBisCO large subunit(precursor) in Gunong13, Fuyi and LTH, RuBisCO large chain precursor in Quanfengand Fuyi, chlorophyll a/b binding protein in Quanfeng and LTH, triosephosphate isomerase in Tiangu and LTH were all down-regulated; We also found some specific proteins in specific rice varieties: such as glycine dehydrogenase in Gunong13, cDNA-DUF538domain containing protein in Fuyi, superoxide dismutase in Tiangu, Clpprotease in Gufeng and fruit protein in LTH were all down-regulated, whilephosphofructokinase in Gufeng and peroxiredoxin in Tiangu were up-regulated.3. The result showed that some proteins were down-regulated in both the ricesterile lines with blast-resistance ability and the control LTH, such as RuBisCO largesubunit (precursor), chlorophyll a/b binding protein, Clp protease, fruit protein and triosephosphate isomerase. While some proteins were regulted with different manner in the two kinds of rice varieties, such as OEC polypeptide, which was down-regu lated in rice sterile lines and up-regulated in control LTH.4. A total of55differential expressed proteins were identified in rice infected with blast, about80%proteins were down-regulation, suggesting that blast infectioninhibited the expression of some proteins, thus affecting the body’s normal metabolism. These proteins could be divided into five groups according to their function:26proteins involved in photosynthesis,14proteins participated in energy synthesis andmetabolic process,5proteins related to stress response,5proteins involved in proteinmetabolism,1protein involved in signal transduction. While70%of proteins involved in photosynthesis and energy metabolism, indicating that blast infection seriouslyaffected photosynthesis and energy metabolism pathway.