Comparative Proteomics Studies On Silkworm Eggs Infected N.bombycis

Microsporidia are unicellular eukaryotes that were obligate intracellular parasitesgenerally exsited in the natural world．Nosema bombycis can cause pebrine disease andlead to destructive disorder,and big deaths in silkworm by vertical transmission,SOdefined as the only disease for quarantine in sericulture．Our country lose up to tenmillion every year because of the disease．disease．Thus how to detect the pathogenearlier and take effective measures to cut down the loss is very urgent in sericuituralsciences． Though there are sevearal detection methods， their defects are alsoobvious．Optical test under microscope are reliable but time-consuming and needprofessional workers．PCR is the predominant means among the molecular biologicaltest techniques， but it need expensive instruments for amplification andvisulization．Thus，how to invent a fast,convenient detection method which Can beapplied in production is a big problem to solve．This experiment with comparative proteomic, and analyses the methods of thepoisonous insect Nosema bombycis silkworm eggs and normal silkworm eggsdifferences protein. Many enzymes showed great differences, including participation incarbohydrate metabolism and the cell cycle part of inoculation enzymes with Nosemabombycis of silkworm eggs on the impact of the trend, such as the origin recognitioncomplex subunit1,UDP-glucosyltransferase, tyrosine phosphatase, nitric oxide synthase,etc, this kind of phenomenon can think of the silkworm with Nosema bombycisinfection make an effective response to involve a great deal of gene expression, thismay require inhibition of growth and shut down those not must metabolic pathways.SDS-PAGE gel electrophoresis and fluorescence quantitative PCR results show that the poisoned with Nosema bombycis silkworm eggs HSP90in high expression,poisoned with Nosema bombycis silkworm eggs HSP90expression in amount close tonormal eggs7times. HSP90’s main function is to maintain stable cell protein, improvethe cells to stress tolerance, enhance the antioxidant effect make cells maintain normalphysiological function. HSP90is the most active molecules in cell one partner, wideparticipation of cell signal transduction, hormone response and transcription regulationprocess, the cells in physiology, pathology, and stress conditions of existence has playedan important role. HSP90in fungal infection plays an important role of the adjustmentfactor, is also many fungal disease detection and treatment targets. With Nosemabombycis infection in silkworm eggs HSP90protein show obvious rise, this kind ofphenomenon can think of Nosema bombycis invasion of the destroyed the normal lifeactivities silkworm eggs, could cause cell apoptosis, but induce produce HSP90so as toimprove the silkworm eggs to the Nosema bombycis tolerated. So HSP90is expected tobecome infected withNosema bombycis silkworm eggs test targets.Silkworm protein as silkworm eggs with target detection has more obvioussuperiority, because with Nosema bombycis gene or protein as hard to improve thedetection test silkworm eggs target of sensitivity, the silkworm eggs with Nosemabombycis relatively small number. And with the protein as target detection silkwormeggs have amplification effect, will greatly improve the detection sensitivity, will bethinking and method of inspection inspection deduction of the major innovation.