Construction Of Solanum Pennellii LA0716Introgression Lines And Mapping Of Related Agronomic Traits

Tomato (Solanum lycopersicum) is one of the most important cash crops widely cultivatedthroughout the world and also an important research material in the biological sciences. During thelong-term process of their domestication from wild species to cultivated species, many useful genesgradually lost because of the constantly intra-specific hybridization, and the genetic base of moderncultivars became more and more narrow. To explore and utilize beneficial genes of wild germplasmresources is of great significance for innovating breeding materials and improving yield and quality oftomato. By utilizing cross, consecutive backcrosses and molecular marker-assisted selection methods,cultivated tomato S. lycopersicum1052and wild tomato S. pennellii LA0716were used to develop anintrogression lines (ILs) which contained the whole genome of S. pennellii LA0716with the geneticbackground of S. lycopersicum1052. The main results were summarized as follows:1、A total of447CAPS and525SSR markers were used to screen polymorphic markers amongtwo parental lines (S. lycopersicum1052and S. pennellii LA0716) and their F1progeny, which resultedin216polymorphic CAPS and236polymorphic SSR markers. The parental polymorphism was46.5%.200molecular markers uniformly distributed over the entire genome were selected to construct thepopulation. The genetic distance between two markers was6.3~10.0cM with the average interval of7.29cM.2、By cross (1times), backcrosses (5times), self-cross (1time) and molecular marker-assistedselection (5times),107ILs were finally obtained from588BC5ILs candidate plants. These lines, whichcovered the whole genome of S. pennillii LA0716(1458cM), contained the average introgressionsegment of31.5cM.3、By cross, backcrosses and phenotypic screening, thirteen near isogenic lines (NILs) harboringdifferential agronomic characters were developed. These traits included fluff, green stems, yellowleaves, sweet potato leaves, dark pulse, green fruit, white fruit, yellow fruit, violet stripes, green stripes,purple fruit, pink fruit and late-mature.4、NILs materials were obtained, which harbored tightly linked markers of various resistance genes,including tomato yellow leaf curl virus resistance gene（Ty1、Ty2、Ty3）, fusarium oxysporum resistancegene（I-2、I-3）, tomato spotted wilt virus resistance gene Sw-5, bacterial speck resistance gene Pto,tobacco mosaic virus resistance gene Tm-2and cladosporium fulvum resistance gene (Cf5、Cf9).5、By indentifying the mapping populations of different agronomic traits through molecularmarker-assisted selection, the yellow flesh gene was mapped in the area of46.5~98.0cM inchromosome6; the netted fruit gene was mapped in the area of95.0~97.5cM in chromosome4; thegreen stem gene was mapped in the area of51.0~56.3cM in chromosome9; the yellow leave gene wasmapped in the area of45.0~77.0cM in chromosome9; the potato leave gene was mapped in the area of67.0~98.0cM in chromosome6; the leaf venation gene was mapped in the area of0.0~33.0cM inchromosome3.