Proteomics Research Of BHK-21Cells Infected With Asia Ⅰ FMDV

Foot and Mouth Disease (FMD) is a severe, highly contagious, vesicular disease of cloven-hoofedanimals including domesticated ruminants and pigs and more than70wildlife species. Generally, thedisease causes25%cattle with FMDV infection losing fertility and high mortality of young stock due tothe myocarditis. Because it has the potential for rapid and extensive spread within and betweencountries, thus, it can cause severe impact to breeding industry and economic development. FMDVinfection can cause changes of cellular proteome within the host cell, which is the result of interactionsbetween FMDV and host cells. The protein is the executive body of the life activities and thefoundament of host cells showing various functions, so it is helpful to use the proteomic methods toresearch the characters and functions of these proteins for revealling the pathogenesis of FMDV and toprovide a theoretical basis for research and development of new type of drug and vaccine.To research the cellular response to FMDV infection, with the BHK-21cells, firstly wemorphologically proved the subcellular changes and the apoptosis of baby hamster kidney cells(BHK-21cells) with FMDV infection through the transmission electron microscope and the flowcytometer. And then, the differential proteome of BHK-21cell, infected and uninfected with FMDV,were analyzed with two-dimensional gel electrophoresis (2-DE) and PDQest tool. Totally alteredexpression of42protein spots were found, of which30spots being identified by MALDI-TOF-MS/MS,including19up regulated spots,9down regulated spots and2viral protein spots. Further we confirmedthe increasing of NME2protein (expressed in non-metastatic cells2protein) by western blot duringFMDV infection. At last, we analysised the subcellular distribution of NME2through the expression ofGFP-NME2fusion protein, and observed that the NME2proteins mainly distribute in the nucleolus ofBHK-21cells.It is the first time to research the pathogenesis mechanism of FMDV using proteomics and thestudy has supplied some available information for additional research.