Preparation Of The Actived Carbon Nanoparticles Adjuvant And Study On The Immnune Effect

Actived carbon nanopartical is prepared from medicinal actived carbon, which is a kind of carbon materials with a strong ability of absorption, long time sustained-release function, better lymph target and high stability. In the pharmaceuticals industry and clinical application it is usually used as a kind of antidote, lymph tracer and drug vector. In this study, we processed the medicinal actived carbon into nano-diameter by the grind mill. Eveluate the main characters basic properties, such as:size, surface area and hygroscopic. We also studied on the absoption ability of some special proteins and polypeptide antigen in order to research its immune effect.1Preparation of ACNP and its basic properties researchWe determined the optimization preparation condition by the orthogonal experiments design. The design investigated four influence factors including grand time, grind speed, raw material dosage, and the ratio of the water and the carbon’s dosage. The size distributin showed that when the time was10h, seepd was500rpm, raw material dosage was10g, and the ratio between the water and the carbon’s dosage was20:80, the minimum average size was244.6±48.73nm.2Preparation of ACNP suspendsIn order to obtain the best dispersion suspend, we used Tween-80, PVP K30, HVCR and CMC-Na as suspending agents, which are usually used in the pharmaceutical industry, and then we also optimized their dosage. The machine here we used was Zetasizer Nano ZS90. Finally, with the0.3%concerntration of the PVP K-30and ultrasonication under the200W power in2min, we can get the well prepared ACNP suspend.3Study on the absorption effect of the ACNPIn this experiment we tried to use the ACNP to absorb the Lasota New castle disease(NDV), bovine serum albumin(BSA), ovalbumin(OVA) and a synthetic peptide of foot-and-mouth disease virus type-0as the absorption models, then the result showed that the ACNP has a more stronger absoption ability, especially compared to the medicinal actived carbon.4Studies on the immune effect as an adjuvantIn this experiment we investigated the effect when the ACNP was used as an immune adjuvant. According to the release experiment of synthetic peptide of foot-and-mouth disease virus type-O in vitro, we find that the ACNP has a slow controlled release function. Then the mouse was vaccined intraperitonealy with0.2mL at a21-day interval, three times in all, and the developed antibodies to the peptide was determined by LPB-ELISA. In contrast, six mouse inoculated with the oil adjuvant vaccine of the developed antibodies were also tested. The result showed that the commercially vaccine could induce to produce a high antibody titer, and the ACNP adjuvant group failed to induce to produce antibody.