Effect Of Vitamins C,E And Electrical Pulse On Canine Oocytes Maturation In Vitro

Objective:this study was to evaluate the effect of Vitamin C, Vitamin E supplementation in culture medium and electric pulses in different field strength on nuclear maturation of canine oocytes in vitro.Methods:0μ mol/L,50μ mol/L,100μ mol/L and200μ mol/L of Vitamin C and Vitamin E were respectively added to the culture medium. The collected canine oocytes were put into the culture medium in randomly. After incubation for48h and72h, the nuclear maturation of canine oocytes were observed. The electric pulses in different field strength were used to effect the the nuclear maturation of canine oocytes.Results:1. After incubation for48h.with Vitamin C supplementation, the ratio of GVBD phsase in200μ mol/L group (14.56%) was significantly higher than the control group and50μ mol/L group (4.44%)(P<0.05), was no any significant difference between the control group (9.44%) and100μ mol/L group (11.05%)(P>0.05). The ratio of GV and MI-MI I phases was no any significant difference in all groups. After incubation for72h the ratio of GV phase in200μ mol/L group (44.96%�'�9.44%) was significantly higher than the control group (25.32%) and was no any significant difference between50μ mol/L group (33.04%) and100μ mol/L group (27.34%)(P>0.05). The ratio of canine oocytes reached to GVBD and MI-MII phases was no any significant difference among all groups (P>0.05).2. After incubation for48h,with Vitamin E supplementation, the ratio of GV in50μ mol/L group was significantly higher than100μ mol/L and200μ mol/L groups (P<0.05). The ratio of GVBD in100μ mol/L group was significantly lower than other groups (P<0.05). The ratio of canine oocytes reached to MI-MII phases was no any significant difference among all groups (P>0.05), but was relatively higher in100μ mol/L group. After incubation for72h, the ratio of GV phase in200μ mol/L group was significantly higher than the control group and100μ mol/L groups (P<0.05), but was no any significant difference between50μ mol/L group(P>0.05). The ratio of canine oocytes reached to GVBD and MI-MII phases was no any significant difference among all groups (P>0.05).3. The ratio of MI-MII phases of canine oocytes in treated groups (10.51％、14.46％、14.36%) was higher than in the control group (6.27%), but was no any significant difference among all groups (P>0.05)in100μmol/L group. After A combination of100μmol/L was significantly lower the proportion of canine oocytes in the stage of GVBD (P<0.05).Conclusions:1.there was no significant promoting effect on nuclear maturation of canine oocytes with Vitamin C, Vitamin E supplementation in vitro.2. the ratio of nuclear maturation of canine oocytes was not significantly elevated by treated with electric pulses in different field strength.