| AIM:Determine the amoxicillin and sulbactam pivoxyl dosing ratio; to establish the method of detection amoxicillin and sulbactam after extraction from the Plasma and tissues of broiler, using high Performance Liquid chromatography (HPLC). And to study on Pharmacokinetics, absorption and distribution of the drugs in broiler. In order to provide evidence for veterinary Clinical medication.METHODS:Identify drug ratio of in vitro antibacterial test; the Plasma samples were collected in different intervals after intravenous of Sulbactam Sodium; The Plasma and tissues samples were collected in different intervals after intravenous and oral administration of amoxicillin and Sulbactam Pivoxyl (4:1). The samples were determined by using HPLC after liquid-Phase extraction and nitrogen dryer adding suitable mobile Phase. The concentration-time data of drugs in Plasma and tissues were analyzed with3P97Pharmacokinetics Program.RESULTS:Determine the two drug ratio of4:1; the correlations of calibration curve were all good, while correlations of coefficient were more than0.9990. Amoxicillin and sulbactam the limit of detection (LOD) were0.00μg/mL,0.05μg/g in plasma and tissues respectively. The average extraction recovery was more than70.17%from the Tissues and Plasma. The inter-day coefficient of variations and inter-day coefficient of Variations were less than5%and10%respectively. The broiler received intravenous Sulbactam Sodium (3.75mg/kg·B·W), The results indicated the Plasma drug concentration-time data was fitted two compartment open models. The disposition half-life (t1/2α) of the drug was0.090±0.001h, the elimination half-life (t1/2β) of the drug was0.919±0.011h, the area under the serum concentration-time curve (AUC) was6.137±0.376βg-h/mL, the total body clearance (CLb) was0.611±0.051L/h/kg. After oral administration Amoxicillin and Sulbactam Pivoxyl (4:1), Amoxicillin15mg/kg·B·W, Sulbactam3.75mg/kg·B·W. The results indicated the Plasma drug concentration-time data of amoxicillin was fitted two compartment open models. The (t1/2α) was0.627±0.071h, the t1/2ka was0.139±0.011h, the t1/2β was1.800±0.181h, the AUC was6.005±0.641μg-h/mL, the time-Point of maximum Plasma concentration of the drug (Tmax) and the maximum plasma concentration (Cmax) were calculated as0.476±0.049h and Cmax2.293±0.152μg/mL. The results indicated the Plasma drug concentration-time data of Sulbactam Pivoxyl was fitted single compartment open models. The t1/2ka was0.087±0.007h, the t1/2βwas2.044±0.165h, the AUC was4.109±0.364μg·h/mL, the Tmax was0.412±0.039h, the Cmax was1.212±0.092μg/mL, the bioavailability (F) was66.96%±5.95%.After oral administration amoxicillin and Sulbactam Sodium (4:1). The study of distribution revealed that amoxicillin, with the exception of kidney medicine at the curve in the two-compartment model; the remaining was in a Single-compartment model of absorption. The curve equation of concentration-time in musele, kidney, liver, and lung were C1=3.4887(e-0.0475t-e-0.5741t), C2=5.0172(e-0.0482t-e-0.6536t), C3=6.8054e-0.1391t+0.8980e-0.0196t-7.734e-0.46241, C4=3.3329(e·-0.0378t-e-1.4421t). The study of distribution revealed that sulbactam Pivoxyl, the curve equation of concentration-time in musele, kidney, liver, and lung were C5=1.5376(e-0.0340t-e-0.8305t), C6=2.1507(e-0.0346t-e-0.8177t), C7=2.7673(e-0.0367t-e-0.8216t), C8=3.0437(e-0.0491t-e-1.4565t). |
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