Functional Analysis Of The Oryza Sativa Phosphate Transporter Gene Famil

Phosphorus is one of the most important nutrients which limit plant growth and development. Inorganic phosphate (Pi) transport through plant membranes is mediated by a number of Pi transporter proteins. Plants regulate the expression of Pi transporter genes to adapt low available P environment by enhancing their ability to acquire and transfer Pi. Other factors in the external environment will also affect the phosphate transporter gene expression and regulation. Rice phosphate transporter protein gene (PT) is a multi-gene family, Different members of these families play different roles in rice growth and development process or in a variety of physiological and biochemical and metabolic processes. Analysis of phosphate transporter gene family in rice research helps us understand the members of gene function in phosphorus uptake by rice and transhipped, signal transduction and phosphorus and phosphorus concentration in maintaining balance and other aspects of regulatory mechanisms in the body, but also for the creation of resources of phosphorus efficient rice, cultivation of phosphorus efficiency of rice varieties to provide a theoretical foundation.This study is comprised of two parts. The first part is phosphate transporter family genes bioinformatics analysis in rice, including rice PT family members, phylogeny, motif and protein structure, cis-acting elements of2-kb upstream region of these genes, OsPT family members after different phosphorus environmental condition as well as different hormone processing the gene expression spectrum analysis, has carried on the confirmation through Real-time PCR to the partial chip result The second part is Phtl family13PT gene overexpression vector as well as two sets of inhibition of expression vector construction, genetic transformation, identification of transgenic plants that create the genetic material for subsequent in-depth study of gene function. The main resultares are as follows:1. Bioinformatics analysis of26phosphate transporter gene in rice, were named as OsPT1、OsPT2、OsPT3、OsPT4、OsPT5、OsPT6、OsPT7、OsPT8、OsPT9、OsPT10、 OsPT11、OsPT12、OsPT13、OsPT14、OsPT15、OsPT16、OsPT17、OsPT18、OsPT19、 OsPT20、OsPT21、OsPT22、OsPT23、OsPT24、OsPT25、OsPT26。2. Phosphate transporter protein bioinformatics analysis revealed that they have eight conserved the motif,5-12transmembrane domains, most domains are conserved.3. A total of237putative cis elements were found in the2-kb upstream region of these genes. Of these, a majority were Pi-response and other stress-related cis regulatory elements, such as PHO-like, TATA-box-like, PHR1, or Helix-loop-helix elements, and WRKY1and ABRE elements, suggesting gene regulation by these signals.4. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from27tissues covering the entire lifecycle from three rice genotypes:Minghui63, Zhenshan97, and Shanyou63.5. Comprehensive expression analysis of these genes was performed using data from microarrays hybridized with RNA from Hormone-treatment experiments of three rice genotypes:Minghui63, Zhenshan97, and Shanyou63.6. The partial chip result after the Real-time examination confirmation, had indicated OsPT gene family various members has the different expression spatiotemporal mode under the normal growth condition OsPTll、OsPT17、OsPT20are preferentially expressed in stamen at1d before flowering, OsPT4、OsPT13are preferentially expressed in flag leaf at14d after the heading stage.7. The paddy rice seedling stage with5kind of hormone processing, examines in the paddy rice PT gene family with Real-time11gene expression quantity, discovered that these genes have the different response pattern to different hormone processing, suggested they the phosphate transportation aspect have the different function in the phosphorus signal transduction variant form8. In the phtl family13PT gene’s over-expression vector as well as2sets suppresses the expression vector the construction, to carry on the genetic transformation in the nipponbare acceptor variety, various carriers obtained10-60about the transformed seedlings, has carried on the masculine appraisal, the expression quantity examination to these transgene adult plant, has formulated the heredity material for the following deep research various genes’ function.