Distribution Of Obestatin In The Gastrointestinal Wall Of Piglets At Different Stages

Obestatin is a new protein found in the bioinformatics analysis of gene sequences of the stomach auxin precursors (Proghrelin), and able to suppress hunger, so it is named obesity inhibin. Ghrelin is involved in weight regulation and able to increase food intake, reduce energy consumption, and lead to weight gain. Obestatin is considered to be biological antagonists of Ghrelin, and has a biological role which is diametrically opposite to Ghrelin, and combines together with the orphan G protein GPR39receptor, leads to inhibition of food intake, gastric emptying, peristalsis of the jejunum and weight gain and therefore has an important biological role in the complex mechanisms of the body’s energy balance and weight regulation.Domestic researches on Obestatin mostly focus on the plasma changes and its effects in human and rat disease models. There are not many studies on pigs, and no relevant literature. Piglet stage has the most rapid growth and development and the most metabolism of pig, and is one of the most important stage in the pig farming. Research of Obestatin in the piglet stage has very important potential value to stress prevention and fattening piglets. Therefore, this experiment tests the distribution of Obestatin in various parts of the gastrointestinal tract of piglets at different stages, to explore the distribution of Obestatin in different stages of the gastrointestinal tract, to provide experimental basis for further study of Obestatin in the piglet digestive system and energy metabolism and in-depth understanding of Law in piglet gastrointestinal mucosa development.In this study, the new-born piglets (1day old), breast-feeding piglets (15days), weaned piglets (30days) and bred pigs (45days) were selected and killed, followed by separation and acquisition of gastric fundus, gastric body, gastricantrum, duodenum, jejunum, ileum, cecum, colon and rectum. Taken tissues were fixed in4%neutral paraformaldehyde for24h immediately, then were made to paraffinembedded tissue. Using SP Immunohistochemical staining kit and DAB chromogenic method, each paraffinembedded tissue was detected by Immunohistochemistry. The test results were photographed and analyzed. The results analysis shows that:(1) Obestatin exists in various vivo gastrointestinal parts of the piglets at different stages and are widely distributed。It densely distributed in the top of the mucosal epithelium, the submucosa and serosa of the entire gastrointestinal tract, and the amount of the existence is large; distribution of Obestatin in gastric glands, the lamina propria, muscle layer in the stomach wall of the stomach and the midgut gland, lamina propria, muscle layer in the intestinal wall of intestinal segment is less than the top of the mucosal epithelium, the submucosa and serosa.(2) In gastrointestinal tract of newborn piglet, the Obestatin distribution in the gastric fundus and colon is extensive and large compared to the one in other parts; In breast-feeding piglets, Obestatin distribution in the gastric antrum and jejunum is larger and broader than the other parts of the gastrointestinal tract; in weaned piglets, the distribution of Obestatin in gastric fundus, cecum, colon is larger and broader than the other parts of the gastrointestinal tract; in conservation piglet, a large number of Obestatin evenly distributed in gastric fundus and gastric body, and mainly in the lateral mucosal epithelium in the gastric antrum, and the distribution of Obestatin in cecum, colon is larger and broader than the other parts of bowel wall.(3) Changing with the age increasing and developmental stages, the Obestatin distribution in the mucosa of gastric fundus, gastric body, duodenum, ileum, cecum, colon and rectal gradually increases; distribution of Obestatin in the epithelium of gastric antrum showed trends "increases first and then concentrated", the distribution of the top epithelium gradually increases, and in the lamina propria and gastric gland, the distribution gradually reduces, and finally the Obestatin distribution in the mucosa are concentrated in the top of the epithelial, less in lamina propria. Obestatin contents of the duodenal mucosa in weaning stage are larger compared with breast-feeding stage and conservation stage; the Obestatin distribution of the jejunal mucosa showed the trend "first increased and then decreased, again resume", Obestatin decreased in the middle stage named the weaning stage; colon mucosal distribution of Obestatin in the conservation stage gets antral-like trends of "concentration", a large number of Obestatin distributes in the top of epithelial, and it is still more Obestatin distribute in the lamina propria.