Proteomic Analyses Of Chicken Kidney Tissues Infected With Newcastle Disease Virus

Newcastle disease virus (NDV) can induce acute and highly contagious disease,For the past fewyears,The prevalence of new castle disease is increasingly complex, Immunifaction failure fequentlytake place,Newcastle disease is still the important disease dangerous to poultry industry.At clinicalresearch,NDV was characterized as having specific property to antineoplastic, Thus modern medicineinvestigation confer more and more attention, Relying on the proteomic technology and molecularbiology,our reseach identified some differertly expressed spots and sort them according to theirsubcellular localization and molecule function. Our purpose was to reveal the relationgship betweenNDV and its host and some underlying pathopoiesis mechanism.This reseach make use of the2-D gel electrophoresis technology to explore differential expressedspots which showed different tendency beween the mock-infected and NDV-infected kidney tissue. Dueto the using of NCBI and UNIPROT SWISS/PROT database and some related documents,We foud thatthese protein are mainly associated with the cystoskeleton, stress reaction, glycometabolism, energymetabolism, apoptosis.the different level at mRNA level of these proteins(apolipoprotein AI,28-kDavitamin D-dependent calcium-binding protein, tubulin beta-7chain,78-kD glucose-regulated proteinprecursor, Annexin V, manganese-containing superoxide dismutase precursor) further validate their2-Dresults. Additionally three proteins (GRP78, ANXA5,28KDA-CALB) was checked by westernblot,indicating that the results of real-time and western was consistent with the2-D results.This research obtaind relatively complete and accurate differential expression data and providedvaluable message to the knowledge of the NDV pathopoiesis mechanism and its relationship to host...