Pathogenic Microorganisms From Aquaculture Sea Cucumber Biological Treatment Technology Research

In recent years, with the sea cucumber aquaculture have been expanding, bacterial diseases of farmed sea cucumber has become a sea cucumber aquaculture industry is facing the most prominent and urgent the problem. At present, the use of antibiotics is an effective method of inhibition of bacterial diseases of the sea cucumber, antibiotics, heavy use of the adverse effects of very serious. Bdellovibrio cracking Gram-negative bacteria has a specific to kill pathogenic bacteria, purify the water. Bdellovibrio in aquaculture research and have received increasing attention, Bdellovibrio as probiotics has no poison, no side effects, effectiveness and stability characteristics. Use the Bdellovibrio instead of antibiotics to control pathogens, showing the huge market demand. Separation of pathogenic microorganisms for identification of marine aquaculture water bodies investigated Bdellovibrio host cracking and pyrolysis conditions, sea cucumbers and other Seafood aquaculture water purification, has important theoretical significance and value.Bacteria isolated from eight samples of57to observe the microscopic morphology and Gram staining. Some strains of the16s rDNA sequencing showed that the sequence alignment, respectively for diesel food methane bacteria Alcanivorax dieselolei: Alcanivorax sp. C13, the Alcanivorax dieselolei H34, Pseudomonas species: Pseudomonas marincola H36, Pseudomonas marincola H37, Tenacibaculum sp.: Tenacibaculum sp. B4, Shewanella baltica:Shewanella baltica strain the S55the Dietz bacteria Dietzia natronolimnaea:Dietzia natronolimnaea Hong, wax like Bacillus cereus:Bacillus cereus B7, Bacillus cereus B8Proof of The greatest similarity (98%). Which marine flex bacteria, Shewanella, and Pseudomonas known sea cucumber epidermis rot disease, the Department of sea cucumber farming pathogenic microorganisms.Investigated the cleavage of the of Bdellovibrio BdeD23part of the sea cucumber aquaculture water bacterial isolates; investigated at various temperatures, and static/shaking culture conditions, BdeD23cracking effect on E. coli JM109.,37℃shaking culture, OD600is drop Bdellovibrio lysis effect of the host bacteria, the most obvious. The article also examines BdeD23lysis of E. coli JM109. At different NaCl concentrations, the concentration of NaCl in the case of less than0.5%or higher than3%are unable to form plaque,1%NaCl, spot the shortest (63h), the fastest pace of expansion of plaque.Conducted a preliminary exploration of this study is to kill the microbial diseases of sea cucumber farming Bdellovibrio confirmed Bdellovibrio sea cucumber breeding and microbial lysis, while identifying the constraints and the level of Bdellovibrio lysis of host bacteria. Bdellovibrio as a new type of probiotics used in microbial disease prevention and control of sea cucumber farming provides a theoretical foundation with practical guidance.