Analysis Of Differential Proteomics On Chililling Stress Response In Brassica Napus L.

Rape is an important oil crop, in most parts of the world have grown. Our country wide planting range, large area, latitude is larger, and our country’s temperate continental climate, easy to make rape encounter frozen disasters, causing great economic loss. Typically rape encounters the cold, frozen disasters, rape by altered morphology, physiological and biochemical process, so that the low temperature stress responsive gene expression, synthesis of specific proteins in response to low temperature stress. In recent years on the low temperature condition, specific protein research has become the proteomics research hot topic, related to wheat, rice, Arabidopsis proteomics research is increasing. However, the rape of proteomics research has seldom been reported, for low temperature stress on rape physiology and biochemical processes that impact on the molecular mechanism is not very clear. So the study of rape under cold stress in protein level changes, find and low temperature stress associated with a specific protein, rape and other crop resistant genetic engineering in the improvement of provide very valuable information, it has the important practical value and economic significance.In this paper, with cabbage type rape gold margin oil No las experimental material, through4degrees and25degrees temperature stress temperature control, the determination of the enzyme activity change rule, using two-dimensional electrophoresis technology research under low temperature stress, leaf proteome changes, by mass spectrometry technology on low temperature induced differential proteins were identified, hope plants as revealed in the low temperature condition in the expression of proteins, for the establishment of cold stress in plants under the conditions of protein molecular networks, eventually shed under low temperature stress in plant cold hardiness mechanism provides a theoretical basis, while searching for and low temperature related protein, for further research on the mechanism of cold resistance in Brassica napus L. foundation.The main results are as follows:(1) after induction of hypothermia after treatment of rape seedling leaves smaller, at the same time, the color yellow, the roots of fine short.(2) through to rape seedlings after different time of low temperature treatment, wherein the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) activity were demonstrated increased first and then decreased trend. Three types of enzyme activity by treatment time and temperature influence.(3) by using differential proteomics strategy, establishment of Brassica napus seedling protein two-dimensional electrophoresis system, scanning electrophoretie pictures, use PDQuest software to undertake analysis, comparison of4degrees and25degrees are not treated seedlings seedlings low temperature processing differential proteins, found in25degree expression protein spots in172,4the expressed protein was190,4degrees after low temperature treatment of protein expression levels in significant up-regulation of protein spots with24, significant downregulation of protein has12point. Only in25degree expression protein spots in2, only in4degree expressed protein20.(4) the use of mass spectrometry techniques successfully identified5differentially expressed protein spots, respectively:glutamine synthetase, ribulose two phosphoenolpyruvate carboxylase, oxygen enhanced protein, photosystem Ⅱ protein, and an unknown protein, main plant and radical scavenging, photosynthesis and photorespiration related.Low temperature stress, rapeseed protein spots marked change, protein expression of up-regulation of protein spots for a total of24, significant downregulation of protein spots for a total of12. Low temperature stress early (1-5d), due to respiration increased, resulting in a large number of superoxide free radicals, forcing the superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) and protective enzyme system of enhanced activity, superoxide radical scavenging activity, in order to reduce oxygen or other peroxide radicals on cell membrane damage; at the same time, ribulose two phosphoenolpyruvate carboxylase, oxygen enhanced protein, photosystem Ⅱ protein was significantly higher in photosynthesis, shows serious suffocate suffocate case, through the above photosynthesis related protein expression of increased responses to low temperature stress. Based on the above results, we think that the chilling stress rape respiration enhanced, reduced photosynthesis, plants through specific changes in protein expression in response to low temperature stress, decreased from low temperature damage. This is from the molecular level to reveal the mechanisms of response of rape seedlings under low temperature and laid a preliminary foundation.