| Rabbit as a miniature plant-eating livestock, mainly with plant feed is given priority to, the digestive physiology to feed themselves a lot of plant cell wall has strong adaptability. Crude fiber in the rabbit in the diet, except as a part of the energy source, the main function is to constitute reasonable fodder structure, maintain normal physiological function of the digestive tract. Rabbit intestinal infection often occur in the YouTu after weaning, easy to cause the death of the rabbit and growth rate of decline. After weaning rabbit intestinal health depends on the diet, the level of lignocellulose and quality. But at the same level of the ADF, because the level of DgF different, after weaning the intestinal health status of great changes. This topic through the different DgF/ADF level of diet to35to70days of age New Zealand production performance, the digestive tract feeding rabb enzymatic activity and the cecum flora diversity, and the influence of microstructure, the more modern micro ecology molecular biology technology means of evaluation of the cecum flora succession law to determine the rabbit in the diet, appropriate DgF/ADF level. For probiotics provide theoretical basis.Test, the choice of New Zealand rabbits by weight similar principles, the single factor experiment design, will only28,192, in strict accordance with age weaning rabbit test design, and test sampling and statistical analysis, determination of different day age feeding rabb New Zealand small intestine amylase activity, trypsin activity, cellulose enzyme activity and the cecum flora diversity. The conclusions are as follows:(1)Different DgF/ADF level of diet,70day age affect weight gain of rabbit are not significant (P>0.05), but for different DgF/ADF ratio of day age group70average day of feed intake, average daily gain weight and material than influence significantly (P<0.05). Experiment with diet, DgF/ADF ratio increases, average daily intake will increase; Average daily gain with DgF/ADF ratio increases after the first increases, reduce Material than with heavy DgF/ADF ratio increases, reduce increased after the first. Test when DgF/ADF=1.3, material than the heavy, on average, the biggest daily gain, were4.17:1and28.63g.(2) Different DgF/ADF level on the digestive tract diet, enzymatic activity affect significantly (P<0.05). Amylase activity to deal with I group as the highest, for15.82U/g, treatment group IV minimum, for13.65U/g, the difference between the treatment group was significant (P<0.05); Trypsin activity in diets with DgF/ADF the increase of the ratio of first increases have down trend, show curve changes; Cellulose enzyme live in diets with DgF/ADF ratio increases, and reduce, the difference between the treatment group was significant (P<0.05), which I treatment groups of cellulose enzyme activity is the highest, about33.43U/g, diet, DgF/ADF ratio influence in the cecum cellulose enzyme activity of rabbit.(3) Different DgF/ADF level of diet significant influence on the diversity of the cecum flora. This experiment with the PCR-DGGE technology tracking120only, weaning the cecum rabbit area of flora the evolution, the construct different DgF/ADF level diet, under the condition of the feeding rabb New Zealand35and70day age16Sr DNA cloned library, analysis to match DGGE advantage with16cloning by16Sr DNA sequence. The results show that DGGE map by simple (weaning) to the complex (56d), reply again simple (63d) to the complex (70d), and the last tend to be stable. â… , â…¡ group35day age most similar DGGE map, with the most advantage spectrum for e. coli;70days of age â…¡ map complex, escherichia coli exist but not the most advantage with spectrum, after weaning maps of low similarity, different DgF/ADF level diet, led to the area of the cecum flora the remarkable changes of structure,63days of age and70day age group â…¡ map deal with complex, advantage, maps are spectral bands similarity tend to stability. Sequence analysis shows that, A11bands and A14and homology of the highest similarity of microorganism training not only76%and66%respectively, and the relationships of the microorganism of the recently had identified the homologous sex also were76%and66%, so the two strip may mean a new species. A1Peptococcus anaerobius similarity with bands for100%, strip A2and Bacterium lacticum similarity is100%, and the bands and Lactobacillus acidophilus A10similarity is100%, and the channel with Optimal coli similarity A13for100%, strip A16and bifidobacterium species for100%similarity, strip A1, A2, the A10, A13, A16, do not belong to the anaerobic bacteria cultivation bacteria. Strip the A8and have identified saccharomycetes similarity of only95%, that may be a new type of saccharomycetes A3. Channel for â…¡, â…¢ A3, â…£ group son rabbit42days of age of channel of the cecum, said MingZai rabbit after weaning a week channel for â…¡, â…¢ A3, â…£ group advantage bacterium group, channel for each son rabbit49A4day age of bands, rabbit, for each group advantage bacterium group, channel for â…¡, â…¢ A6, â…£ group son rabbit49of the cecum day age of strip, and for each advantage bacterium group, channel for each son rabbit A856day age of bands, rabbit, for each group advantage bacterium group, channel for the A10wang rabbit63day age of bands, rabbit, for each group advantage bacterium group, channel for each son rabbit A1570day age of wang rabbit strips, the advantage bacterium group for each group.In summary, the different DgF/ADF level of diet to35to70days of age New Zealand production performance, the digestive tract feeding rabb enzymatic activity and the cecum flora diversity has significant effect. DgF/ADF the lower level of the diet, can promote the rabbit of nutrients to digest absorb, and reduce the rabbit the mortality after weaning, the health benefits of rabbit, but DgF/ADF at low levels will cause the cecum microflora of imbalance and the cecum flora diversity is reduced, the increase of harmful bacterium, the advantage bacterium group of disorders. DgF/ADF high levels of diet, make the digestive tract enzymatic activity enhanced, but for the balance of the department of the region have adverse effect, reduce diversity, leading to the occurrence of intestinal disease of rabbit, production performance decline. Therefore, the actual production, we must make the rabbit diet, DgF/ADF remained in an appropriate level, only then can let the rabbit get the best production performance, the disease incidence minimum, this experiment, the35to70days of age New Zealand in diets of suitable feeding rabb DgF/ADF level of1.3, in this level, can maintain the best of the microbial flora of balance, the optimal production performance and digestive enzyme live. |
