The Expression Profiling Of Rice PP2Ac Type Phosphatase Proteins In Seedlings Under Salt-stressed Conditions

Background: Rice is one of the most important food crops in world, one of the basic research model plants. Of large-scale production of rice caused by salt damage, the impact of salt stress, involving almost all plant physiological and biochemical processes, leading to serious plant can not grow normally, cuts or even crops. Against salt stress mediated salt adversity molecular mechanism of rice production is of great significance. The PP2Ac class of protein phosphatase (phosphatase 2A catalytic subunits, PP2Ac) is widespread in plants and animals have been shown that rice PP2Ac family members play an important role in the process of growth and development and resilience. Protein phosphatase catalyzed dephosphorylation reaction, so that protein phosphorylation and dephosphorylation, terminate the transfer of signaling molecules, and thereby regulate cell signaling signal transduction pathway. There is evidence that protein kinase and protein phosphatase as a pair of switch regulation of cell within the range of biochemical reactions.Objective: Preparation of the corresponding specific antibodies against rice PP2Ac gene encodes a protein, and then explore the molecular mechanism of salt tolerance of rice for the understanding of an important protein expression patterns, as function of accumulated data.Materials and Methods: The candidate gene encodes a protein epitope prediction, to elect the antigenic determinants of the fragment cluster peak higher, synthetic peptides coupled immune rabbit polyclonal antibody. Detected by Western Blotting PP2Ac genes encoding proteins in rice super hybrid rice parent varieties 93-11 and salt varieties Lan Sheng expression of seedling salt treatment at different time points after the change in resistance by the control reaction in the expression of differentially expressed and transcription of leaf seedling stage in rice MPSS database for comparative analysis.Results: Found in the salt-tolerant rice varieties Lan OsPP2Ac-4 up regulated in salt-sensitive rice varieties 93-11 OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 expression also occurred in the increase, but OsPP2Ac-4 down regulation of PP2Ac protein expression, comparison between the two species and found that PP2Ac protein expression in normal growth conditions, no significant difference remained constant, the expression change occurs only under salt stress. Seedling salt stress transcription data provided by the analysis of the rice MPSS database found OsPP2Ac-2, OsPP2Ac-3 and OsPP2Ac-5 transcription under salt stress levels down.Conclusion: The expression and phenotypic analysis of rice varieties Lan Sheng and 93-11 salt stress on OsPP2Ac process, most OsPP2Ac family in no modification of the expression of salt-tolerant material Lan Sheng, 93-11 in Salt highly expressed within 0.5h, and with the treatment time was gradually increased, peaked at 48h, then the plants die, PP2Ac expression and non-salt-tolerant plant tolerance to salt stress has relevance. More resistant to protein differentially expressed by the reaction and found that the presence of protein is closely related to the expression changes its function. At the same time differentially expressed OsPP2Ac-4 Lan Sheng and 93-11, may provide clues to reveal salt pathway. This experiment found that the four PP2Ac protein expression changes in salt stress, and provide valuable clues for the understanding of the PP2Ac class phosphatase protein function in salt stress in rice and salt-tolerance mechanism.