| This test is designed to the tocolysis effect and mechanism of protective effect of traditional Chinese medicine on animal noise stress induced abortion. In this paper, two-part experiment to explore the the tocolysis effect and mechanism of traditional Chinese medicine on animal noise stress induced abortion.First, the noise-induced mouse abortion model. Pregnant mice were randomly divided into six groups. A group of mice conventional breeding, the blank control group; B, C, D, E and F mice pregnant 3d day, each group, respectively, with 120 dB (The Decibel, dB), 100 dB, 80 dB, 60 dB and 40 dB noise stimulus 5h, for 7 days. Each group of test mice sacrificed in pregnancy 10d recorded embryo development, embryo implantation number and number of dead embryos, and calculate the test groups, the abortion rate, embryo resorption rate. The results showed that the mice in each group due to noise can cause abortion. Group A, the abortion rate was 0.0% embryo resorption rate of 5.49%; group B, 120dB cause the vast majority of pregnant mice abortion, pregnancy, autopsy, no fetus within the uterus, unable to calculate the number of stillbirths; group C, 100dB embryo resorption rate of 92.77%, the first 10d of pregnancy the hepar 100% of the pregnant mice abortion. Group D, Group E and Group F abortion rate is 50%, 20% and 10.0%, embryo resorption rate is 44.83%, 8.89% and 6.52% respectively.Mice pregnant 3d to the first 8d, daily use 100dB noise stimulate 5h 10d necropsy to determine the mouse abortion model for noise-induced noise stimulus intensity and stimulation time Second, traditional Chinese medicine prevention of noise induced in mice and mechanism. Will become pregnant mice were randomly divided into six groups. GroupⅠwas the normal control group, group II noise model group. II, III, IV,ⅤandⅥgroup in the mouse pregnant the first 3d day, each of 100dB noise stimulus 5h, for 7 days, each group of test mice sacrificed in pregnancy 10d. I, II group of mice in pregnancy 4-8d daily fed with the PBS 0.4mL; III, IV andⅤVI mice was pregnant the first 4-8d per day, respectively the irrigation Shichangpu decoction, quercetin + bornyl acetate liquid skullcap + Atractylodes and TCD decoction 0.4mL. Were sacrificed by cervical dislocation in all test rats were pregnant 10d after enucleation bloodletting. Abdominal cavity was opened, exposing the uterus, and observation of uterine growth, and record the number of implantation of the embryo and the embryo resorption of the number of dead embryos. An intact uterus removed in the ice box, cut around the fat and appendages, weighed, and then cut open the uterine cavity vertical stripped fetal membranes and then weighing the uterus weight. After the cut of the right side of the uterus weighing, adding six times the volume containing the protease inhibitors 0.75μg/mL pH7.4 phosphate buffer immediately in ice water environment in the uterine tissue homogenates, 4°C, 12 000 rpm, then centrifuged for 15 min. Remove the supernatant of the packaging, and stored at -80℃ultra-low temperature freezer for the determination of IL-2 and IFN-γ, IL-4, IL-10 and NO.The results showed that traditional Chinese medicine mice due to noise can cause abortion pregnant mice play a tocolytic effect. Noise impact of groupⅠ, the abortion rate was 0.0%, embryo resorption rate of 6.45%; group II 120dB cause the vast majority of pregnant mice abortion, pregnancy necropsy, no fetus within the uterus unable to calculate the number of stillbirths. III, IV, V, VI mice was pregnant the first 4-8d per day, respectively irrigation Shichangpu decoction, quercetin + bornyl acetate liquid skullcap + Atractylodes and TCD decoction 0.4mL The abortion rate was 50.0%, 80.0%, 50.0%, 30.0% embryo resorption rate was 48.15%, 72.94%, 50.00%, 13.18%. The the gramineus decoction, skullcap + Atractylodes, and the TCD group mice abortion rate, embryo resorption rate was significantly higher than in normal controls (p <0.05), quercetin + bornyl acetate liquid mouse abortion rate, embryo resorption The rate of normal controls, no significant difference (p> 0.05). III, IV, groupⅤdifference was not significant (p> 0.05) and groupⅥsignificant difference (p <0.05).Chinese medicine mice uterine tissue homogenates of IL-2 and IFN-γ, the content of NOS activity is higher than the normal control group and lower than the noise model group.GroupⅢ(Shichangpu decoction) and groupⅤ(Scutellaria + Atractylodes) mouse uterine tissue homogenates of IL-2 was significantly higher than theⅠnormal control group (p <0.05) and below II model group (p <0.05 ) between the two groups compared to no significant difference (p> 0.05).Ⅳgroup (quercetin + bornyl acetate liquid) than in groupⅠ(normal control group), the difference was extremely significant (p <0.01) and slightly lower than the model groupⅡ, no significant difference (p> 0.05), andⅢandⅤgroup significant difference (p <0.05) and the theⅥdifference highly significant (p <0.01). GroupⅥ(self TCM group) slightly higher than theⅠnormal control group, compared with no significant difference (p> 0.05), and lower than the model II group differences highly significant (p <0.01). The same of IL-4, IL-10 in mouse uterine tissue homogenates content is lower than the normal control group and higher than the noise model group. TCD groups were significant differences (p <0.05).Conclusion: This study in mice induced abortion with noise, the decibel increase, the mice of the abortion rate and embryonic loss rate was gradually increased so that most of abortion, embryo resorption rate in mice, abortion mice body of Th1 / Th2 balance biased Th1-type response, resulting in immune rejection of mouse embryos induced absorption, the role of abortion, pregnancy failure caused by the production of common endocrine disorders, immune disorders, it is a good experimental model. Gramineus decoction, quercetin + bornyl acetate liquid skullcap + Atractylodes and TCD rate of abortion, embryo resorption rate in the control group, noise-induced abortion miscarriage. |
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