Genetic Analysis Of The Blue-eyed Mutant Of The White Rex Rabbits

This study aimed to inspect and verify the mode of inheritance of blue eyescharacter which found in white rex rabbits.The3mating experiment—crosstest,backcross test and selfcross test were performed. Cross test was divided into twosteps, we selected two white male rex that were labeling as and â…¡as the male parentand5chinchilla meats as the female parent in the first step,respectively,marked as No.1,2,3,4,5,The hybrid combinations were set as×1,×2,×3and Ⅱ×4,Ⅱ×5.Observed the separation of the coat color and hair type on the F1offspring. Cross testhad1blue-eyed mutant of male white rex rabbit and8female rubby-eyed white rexrabbits in the second step, the proportion of male to female were1:8. The blue-eyedmutant of male parent strain was marked Ш, and the8rubby-eyed white rex rabbits offemale parent strain were marked1₁,1₂,1₃,1₄,1₅,1₆,1₇,1₈. Observed the separation of thecoat colors,eye colors and hair type on the F1offspring. backcross test had2blue-eyedmutant of female white rex rabbit which came from Ш×1₁, Ш×1₂, marked as No. B1,B2, Observed and recorded the segregation of F1on their coat colors and eye colors.selfcross test had4blue-eyed mutant of white rex rabbit which came from Ш×B1,Ш×B2, respectively,marked as No. C1,C2,C3,C4. Observed and recorded thesegregation of F1on their coat colors and eye colors.From the assumption of vv homozygote produce the blue-eyed mutant, wespeculated that the genetype of rabbit â…¡was＿aB＿cc＿d＿＿Vvrr; the genetype ofrabbit four was＿aB＿Cchc＿d＿＿Vv Rr; the genetype of rabbit Ш was＿aBbCchc＿dEDevvrr; the genetype of rabbit1₁,1₂,1₃,1₄,1₅was＿a＿＿cc D＿＿＿Vvrr,＿a＿＿cc D＿＿＿Vvrr,＿a＿＿cc＿d＿＿VVrr,＿a＿＿cc D＿＿＿VVrr,＿a＿＿cc＿d＿＿VVrr, respectively; The genetype of rabbit1₆,1₇,1₈all could be AA＿bcc＿d＿eVVrr or Aat＿bcc＿d＿eVVrr or atat＿bcc＿d＿eVVrr;the genetype of rabbit B1, B2had one hundred and twenty-eight type of genes, thesegenes contain vv homozygote. through backcross test and selfcross test, we verified that when the v is homozygosis, it is recessive epistasis towards other genes whichalso control the coat color （A, B, C, D, E）, vv homozygote produce the blue-eyedmutant.