Studies On Transformation Of Genes Into Brassica Napus By Agrobacterium-Mediated

Rapeseed is a kind of the important oil-bearing and economic crops all over theworld, and rapeseed oil is already become the important source for human cooking oil, theyield and quality impact its economic value importantly. Rapeseed is not only the mainsource of edible vegetable oil, but also the potential protein source for large foragefollowed with soybean, and it is the ideal raw materials of the biodiesel used to solve theenergy crisis.The severe drought disaster has appeared in our southwest in recent years, which wasnot occur even in a hundred yearsand which influenced the production of rapeseedseriously and, to a certain extent, the economic benefits of rapeseed. Therefore, with moreand more important position of rape in people’s live, the crop production become more andmore attentioned by people, improving the rape oil content and the quality of rapeseed oil,is already turn into the main target of rapeseed breeding.So, this study using the hypocotyls, callus and inflorescence of the rape to transformthe genes of AtWRKY57, OSWRKY45,BrACCD by agrobacterium-mediated, and detectionthe kanamycin-resistance plants through PCR, RT-PCR, Northern blotting and charactersto get transgenic plants, which can provide technical support and breeding materials forimproving rape varieties by transgenosis. Get the following results,(1) Transformation of OSWRKY45using hypocotyls from xiangyou15and huayou7into Brassica napus by Agrobacterium-mediated and get the transgenic plants.Transgenicplants have more drought-resistance than non-transgenic plants.(2)Transformation of AtWRKY57into yunnan small rape27through inflorescence byAgrobacterium-mediated, and get transgenic plants. Transgenic plants have more drought-resistance than non-transgenic plants. Moreover, build the genetically modified methodthrough inflorescence preliminary, for expending transgenic scale and improving rapevarieties provides conditions. (3) Transformation of BrACCD using callus from xiangyou15and huayou7intoBrassica napus by Agrobacterium-mediated and get the transgenic plants. Improve the oilcontent of the rape and its economic benefits as oil-bearing crops. Transgenic plants ofBrACCD gene has a different oil content increased than nontransgenic plants the, theincrease rate as high as14.6%.