| The experiments were conducted to study the influence of protein content in the feed togrowth performance, feed utilization, activities of digestive enzymes, fish composition of theingredients and serum routine biochemical of juvenile Koi Carp by fed with different proteincontent of feed. Proven the juvenile suitable protein requirement to provide a theoretical basis forlarge-scale farming. The results are as follows:1. The influence of growth performance and feed utilization to juvenile Koi Carp.When the protein content in the range of30.85%to40.57%, juvenile Koi Carp’s survival rate,weight gain rate and specific growth rate increased with increasing of protein content in the feed,they all reached maximum value93.33±7.64%,192.71±13.86%and1.92±0.02%at the proteincontent of40.57%; when the protein content in the range of34.18%to44.05%, fatness increasedalong with the increasing of crude protein content, and obtained the maximum2.42±0.06as longas protein content44.05%, when protein content more than44.05%, fatness began to decreases;protein content in the range of30.85%to44.05%, liver somatic index droped with the increasingof crude protein content, when over44.05%, liver somatic index started to rise again.2. The influence of body composition to juvenile Koi Carp.In the end of the trial, moisture of the juvenile Koi Carp lower than the beginning, reduced inthe range of3.93%to7.28%, which the G-5group was the highest69.56±1.01%; fish crudeprotein content significantly improved to the beginning of the test, show the trend of increasing atfirst and then decreasing with the increasing of feed protein content; crude fat and crude ashcontent were significantly improved,compared to the basis of the control group, crude fat contentshowed a trend of increasing at first and then decreased with feed protein content increasing; eachgroup’s ash content was positively correlated with the protein content in the feed, increased withincreasing protein content.3. The influence of activities of digestive enzymes to juvenile Koi Carp.Koi Carp’s digestive tract protease content in the liver was the least87.36~92.48U g-1, mostin the midgut135.93~169.43U g-1, the foregut protease activity was99.46~117.67U g-1betweenthe liver and midgut. Lipase activity was not obviously different in the liver3.29~4.87U g-1,foregut3.63~4.79U g-1, midgut3.55~6.98U g-1. Amylase activity was not obviously different in the liver41.15~42.53U g-1, foregut39.16~40.31U g-1, midgut38.12~40.87U g-1. Overall, protease,lipase and amylase, these three had the same trend basically, increased at first and then decreasedwith the increase of protein content in the feed, and there were significant differences.4. The influence of physiological and biochemical serum indices to juvenile Koi Carp.With the improvement of protein content in the feed, juvenile Koi Carp’s physiological andbiochemical serum indices were increased at first and then decreased in general, G-4group’s bloodsugar level5.79±0.96mmol L-1was significantly higher than the other group (p <0.05); G-3group’s triglyceride content2.26±0.69mmol L-1was the highest; G-4group’s total cholesterol8.31±0.15mmol L-1was the highest, and significantly higher than that of G-1group5.94±0.65mmol L-1(p <0.05); G-6group’s albumin level was the highest17.4±1.7g L-1, and there was"Alice tail phenomenon"; while the G-4group’s globulin was the highest24.5±3.1g L-1,differences between groups were not significant (p>0.05); G-3group’s AST activity1740±121U L-1was significantly higher than the other groups (p <0.05); G-6group’s ALT activityis the highest79±11U L-1, overall the trend is still increased at first and then droped. |
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