Study On Genetic Diversity Of Siniperca Chuatsi Sensu Lato In The Pearl River

S.chuatsi sensu lato (Sinipercinae:Serranidae:Perciformes), were rare freshwaterfishes and endemic in East Asia. They had important economic value, ecologicalvalue and scientific value. S.chuatsi sensu lato for its meat And refine, delicious intaste good taste and eutrophy, by the general consumers’ welcome.For a long time,affected by natural factors and Human factors, S.chuatsi sensu lato of the mandaringenetic diversity decreasing sharply. on the one hand, unusual climate change,geological disasters destroyed S.chuatsi sensu lato habitat;on the other hand,overfishing, artificial breeding, environment pollution and built DAMS. Not onlymake S.chuatsi sensu lato individual fell rapidly, germplasm resources to sabotage,and affect different groups of communication between genes. Not only the pearl river,in the Yangtze river basin S.chuatsi sensu lato is also facing a genetic diversity of thedecline of the serious trend. As the east Asia region important special economic fish,S.chuatsi sensu lato in genetic diversity protection is urgently needed.This paper, based on the mitochondria cytochrome b genes, Sum to126S.chuatsisensu lato in the pearl river water system and its four main tributaries Geneticdiversity analysis. Results were as follows:1. Genetic diversity: In126S.chuatsi sensu lato from7geographical populationsof the pearl river,16haplotypes were found. All the group showed high haplotypediversity (Hd is0.773) and low nucleotide diversity (Pi is0.00195). Indicating thatS.chuatsi sensu lato populations may have experienced a recent bottleneck or foundereffect, as sufficient haplotype diversity could be accumulated in short-term bymutation, but it were not enough to accumulate nucleotide diversity. In S.chuatsisensu lato populations the highest genetic diversity were found in Liu zhou population,and the lowest were in San jiang population. This may be because of the complexgeological structure sanjiang region, fish life environment is bad, Liuzhou region andregional stability is good,and in liujiang mainstream, With other groups to geneexchange convenient 2. Genetic differentiation: In Neighbor-joining tree based on mtDNA Cytbsequences, different geographical population in S.chuatsi sensu lato didn’t showobvious geographic cluster phenomenon, show that no obvious geographic structure.The genetic distance was form0.001to0.002within groups and was0.002betweentwo of them. Analysis of molecular variance(AMOVA) indicated that8.40%variations were found among groups, and only88.04%variations existed withinpopulations. Showed that the differences between different geographical populationsin the Pearl River are mainly within group.7geographical population in the pearlriver water system, Sanjiang group and other groups all happened between differentdegree of genetic differentiation, this may be due to the total tributaries in sanjiangupstream, away from the mainstream, and sanjiang geographic environment is bad, Sohindered sanjiang group with other groups of communication between genes.3. Population expanding: Mismatch distribution,Tajima’s D and Fu’s Fs neutraltests showed that historical population expansion only occurred in sanjiang populationand liuzhou population, but S.chuatsi sensu lato as a whole didn’t occured populationexpansion. The population expansion of sanjiang population and liuzhou populationappeared in15~400000years ago、18~450000years ago respectively.