The Research Of Separation/Enrichment Chlorophenols Endocrine Disrupitng Chemicals In The Environment By Ionic Liquid Aqueous Two-Phase Extraction/Solvent Sublatlon

Chlorophenols （CPs） are chemical materials which commonly used in pesticides,preservatives and herbicides. CPs are high toxicity and biodegradation is difficult. They canbe enriched through the food chain in vivo. And they are carcinogenic, teratogens, mutagenic.CPs is a priority control pollutants in environmental protection departments. At present, themeasuring method of CPs are maily high-performance liquid chromatography （HPLC）,ultraviolet spectrophotometry, gas chromatography （GC） and gas chromatography-massspectrometry, etc. As the complexity of the sample matrix, which require pre-separation andpre-enrichment before the determination, pre-processing methods are commonly used bysolvent extraction, solid phase extraction, solid-phase micro-extraction, liquid-phasemicro-extraction and liquid-liquid micro-extraction method, and so on. However, thesemethods required the use of organic solvents, lead to secondary pollution. Therefore,establishing a simple, safe, efficient, non-toxic sample separation and enrichment method isvery necessary.In this paper, the ionic liquid aqueous two-phase extraction/solvent sublation systemwere established. CPs in the environment were separated/enriched. The ability of ionic liquid[Emim] DMP and different salts were discussed. The phase diagram data of [Emim] DMP andsalt at different temperatures were measured. In the article, separation/enrichment CPs byionic liquid aqueous two-phase extraction has not been reported. It is a green extractionsystem, which is short time of phase separation, low viscosity, safe and non-toxic. And this isthe first time to propose ionic liquid aqueous two-phase solvent sublation that was used indetermination of trace/ultra-trace CPs in environmental samples. The method has someadvantages that a high enrichment factor, no emulsification, good selectivity, with less organicsolvents, etc.The main conclusions are as follows:（1） The formation mechanism and the abilities of ATPE were studied. The abilities of thedifferent salts for phase separation follow the order K₃PO₄> K₂HPO₄> K₂CO₃.（2） Binodal data for [Emim]DMP+salt （K₃PO₄, K₂HPO₄, and K₂CO₃）+H2O systemswere experimentally determined at different temperature. The phase diagram was drawed. The two-phase area expands with a decrease in temperature, whereas the slope of the tie linesslightly decreases with an increase in temperature.（3） Chlorophenols endocrine disrupting chemicals in the environment were determinedby ionic liquid aqueous two-phase extraction. The highest extraction efficiency for2,4-DCP,2,6-DCP and4-CP were obtained as concentration of NaH₂PO₄aqueous, pH value, extractiontime and the amount of ionic liquid were0.5g/mL,4.0,4min and2mL, respectively. Goodrecovery results （90.2¹07%） with relative standard deviation ranging from1.2%to5.2%（n=5） were obtained when this method was applied to determine these three chlorophenols intap water, lake water, waste water of chemical industry.（4） Chlorophenols endocrine disrupting chemicals in the environment were determinedby ionic liquid aqueous two-phase solvent sublation. The highest sublation efficiency for2,4-DCP,2,6-DCP and4-CP were obtained as the water sample at pH10.70, and subletedwith nitrogen at a flow rate of35mL·min^-1, for40min. With the standard addition method,values of recovery were found in the range of90%～102%, with values of RSD’s （n=11）ranged from1.5%to4.3%.