Study On Tuna Biological Preservation Technology

Penaeus Vanmamei is widely reared in China, but their by-products are not fully used.Optimization of the organic solvent and supercritical carbon dioxide extraction of oil fromHead and Shell, analysis of fatty acid composition of the Penaeus Vanmamei oil extractedand determination of the nutritional value and antioxidant activity of the oil were studied inthe paper.The effects of independent parameters, including temperature, extraction time, solventvolume on the yield of oil were studied, response surface methodology (RSM) was appliedto analysis the independent parameters and their interacted on the oil yield. The statisticalanalysis show that the temperature was the most significant variable followed by solventvolume, extraction time. The optimal extraction condition for oil yield was temperature62.5℃, extraction time3.5hours, solvent volume7mL/g. Under this condition, theextracting rate of Penaeus Vanmamei oil was measured about6.8%.A response surface method was applied to optimize the extraction yields produced bysupercritical CO2extraction (SCE). Operation at optimal conditions (250bar,50℃and20%ethanol) produced Penaeus Vanmamei oil yield6.14%. Extraction pressure has amajor linear effect on oil yield, whilst temperature and time have a lesser impact. Theaddition of ethanol increased extraction efficiency of SCE. The operation at150bar,40℃and20vol%ethanol hd nearly the same yield as that of optimal conditions. This findingallows extraction operated at low pressure and temperature.Fatty acid composition of Penaeus Vanmamei oil obtained was analyzed by GC/MS.The oil were main composed by the five kinds of fatty acids, palmitic acid, oleic acid,linoleic acid, stearic acid, eicosapentaenoic acid, etc. Unsaturated fatty acid content in Solvent extraction was more than the supercritical CO2extraction.The oil extracted bysupercritical on the physical and chemical properties and DPPH scavenging activity wasbetter than Solvent extraction, but the oil color, peroxide value, acid value equivalent werestill too high and needed further processing refining.