Detection Method Of16Particular Polycyclic Aromatic Hydrocarbons In Groundwater By High Performance Liquid Chromatography

Polycyclic aromatic hydrocarbons (PAHs) are well-known and a group of environmentalpollutants all over the world, which is of cancerigenic, teratogenic and mutagenic capabilities.In recent years, PAHs have been detected in the atmosphere, water and soils. PAHs not onlypollute the environment but also cause actual harm to human health, which are so widedistribution that they have been paid more and more attention by people.16of PAHs have beendesignated as detection items firstly by Environment Protection of American(EPA). Othercountries have regarded PAHs as virulent and foul items to be monitored and prevented.7ofPAHs have been designated asdetection items in China. The drinking water and groundwaterthat are closely related to daily life are contaminated by the industrial wastewater including thePAHs. Consequently, it is very important that we must accurately test the concentration ofPAHs in the drinking water and groundwater in order to protect human health. Although someresearchers has developed determination approaches on PAHs, there are still difficulties inaccurate and sensitive analysis of PAHs because of their low solubility of in the groundwater.These become the main purpose of this dissertation. The objective of thesis is to developaccurative and sensitive method to determine PAHs in the groundwater, which may be appliedto determine the content of PAHs in it.16specific PAHs published by the EPA are chosen as determination items in thisthesis, i.e.,naphthalene, acenaphthylene, acenaphthene, fluorene, phenanthrene, anthracene,fluoranthene, pyrene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h,i)perylene, indeno(1,2,3-cd)pyren.The testing procedure of PAHs in groundwater is optimized from sampling, sample preservation, enrichment and purification, concentration and determination aspects.16specific PAHs in groundwater are extracted by using n-hexane liquid-liquid extraction,then through rotary evaporation and acetonitrile phase changing method. Extracted liquidcomparing with the pre-treatment conditions(rotary evaporation and nitrogen concentration) isoptimized. Using High Performance Liquid Chromatography (HPLC) of Waters e2695determines PAHs. Special column for analysis(Waters PAH C18) is adopted. Acetonitrilewater is used to elute. the diode array detector (PDAD) and fluorescence detector (FLD) areused to examine separation and measurement effect. Through the qualitative and quantitative analysis on16standard samples of PAHs inexperimental conditions, the results show that the detection limit is between1.00ng/L-10.0ng/L. The labeled recovery rates of all samples are between85.8%and108%. The RSD is1.86%-9.88%. This method can satisfy the determination demand on the PAHs ingroundwater.