Study On The Acidified Extraction And Purification Of The Flavonoids From Apocynum Venetum Leaves

Apocynum venetum leaves is a species of the dry leaves buds of Apocynumvenetun L. As flavones is the active ingredients of natural produents, now it is playingan important role in antidepressant, anxiolytic, blood pressure and other functions.A series of experiments were carried out to determine the optimal concentration ofhydrochloric acid in the Apocynum venetum extraction solution1. Analyses of the chemical compositions from A. venetum leaves.A high performance liquid chromatography coupled with mass spectrometry wasused for the analyses of the chemical compositions, ten flavonoids were obtained.2. PC12cells effection of antidepressant activity from A. venetum leaves.The activity evaluation systems of pc12cells were established to evaluate theprotect effect of the samples extracted by ethanol aqueous with different HClconcentration.3. Extraction of total flavonoids from Apocynum venetum leaves:Extraction rate of total flavonoids as an indicator, considering solvent extractionand extraction methods, and herbal medicine powder level. Finally, reflux extractionmethod was chosen and optimization.4. Purification of total flavonoids from Apocynum venetum leaves:The absorption and desorption of macroporous resin, considering sampleconcentration, ethanol concentration, and parsing time as the effection on the recoveryrate of flavonoids.