Research The Separation And Purification Conditions Of Methyl Arachidonate By Simulated Moving Bed Chromatography

Arachidonic acid （ARA） is an essential functional fatty acid, the high purity ARA as nutrition enhancer,health care products and pharmaceutical raw materials involved in the regulation of the body’s physiologicalfunction. The purpose of this research is to establish a continuous and efficient process for the separation andpurification of ARA, to obtain high purity ARA products. The microbial oil was raw materials in the test.Through microbial oil pretreatment, D72-Ag⁺resin column separation and purification methyl arachidonateand Simulated Moving Bed Chromatography separation and purification methyl arachidonate. Explored theseparation and purification of methyl arachidonate, optimized process to obtain high purity of ARA. The studyprovided technical support for the industrial production of ARA.1. Microbial oil by saponification, urea clathration, BF₃-CH₃OH esterification, the components ofmicrobial oil fatty acids methyl were analyzed by GC-MS, and the relative content of each fatty acid werecalculated. The results indicated that microbial oil fatty acids included palmitic acid （6.87%）, linoleic acid（19.04%）, stearic acid （7.96%）, arachidonic acid （46.64%）, docosanoic acid （6%）,（n）-tetracosanoic acid（9.02%）.2. Methyl arachidonate was separated and purified from microbial oil methyl by adsorption on D72resinloaded with silver ion chromatography column. Determine the best process parameters of separation andpurification. The methyl arachidonate content was measured by gas chromatography method. Maximumsaturation adsorption amount of the D72-Ag+resin was9mg/g dry resin. In the adsorption temperature of0℃, the loading concentration of unsaturated fatty acid methyl ester sample was5mg/mL, solution of5%acetone in n-hexane （volume ratio） as eluent, desorption temperature of30℃, the elution rate2mL/minconditions methyl arachidonate purity of89.4%, the yield reached79.3%.3. Simulated moving bed chromatography was used to separate methyl arachidonate from Microbial oilmethyl. Methyl arachidonate purity and yield of indicators to optimize the separation process. Experimentaldetermination of the optimal sample concentration was20mg/mL, switching time was720s, and identifiedthe changing range of flow rate of feed speed, elution solution speed, desorption speed, regeneration speed.Used orthogonal combination experiments to optimize process methyl arachidonate by separated andpurificated with continuous chromatography. The optimum parameters were: feed speed39.3mL/min, elutionsolution speed8mL/min, desorption speed4.5mL/min, regeneration speed5.5mL/min. The regional optimalallocation and connection were: eight series connection in the adsorption district, fore series connection in theElution zone, five series connection in the desorption stage, three series connection in the regeneration stage.Under this operating condition, the purity and yield in the final product were91%and92.9%.