Study Of Chmical Constituents And Aioactivity Of Euphorbia Fischeriana Steud And Peper Flaviflorum C.DC.

In this paper, we investaged Euphorbia pilosa Linn, and Piper flaviflorum C.DC to explord their chemiacal constituents and food active constituent. Thirty-five compands were isolated from Euphorbia pilosa Linn, and Piper Jlaviflorum CDC by thin-layer chromatography (TLC), high performance liquid chromatography(HPLC), preparative thin layer chromatography (PTLC),repeated column chromatography (CC) on silica gel, Sephadex LH-20. And structures of22compands in35were determined by modern analysis of spectroscopic methods including1H NMR、13C NMR、DEPT、1H-1H COSY、HSQC、HMBC、IR、ESI-MS、HRESI-MS、UV. The cytotoxic activity of some compounds was tested by means of MTT.Twenty-two compands isolated from Euphorbia pilosa Linn, and eighteen compands were idtentified including3new compands, named as euphopilososide A(1), euphopilososide B (2) and euphopilosolide (3). The known compands were jolkinolide A(4), jolkinolide B(5),17-hydroxyjolkinolide A(6),17-hydroxyjolkinolide B(7), ent-3,14-dioxo-16-atisene (8), phorbol(9), langduin A(10),13-hydroxy-ent-abiet-8(14)-en-7-one(11),2,4-dihydroxy-6-methoxy acetophenone4-O-β-D-glucopyranoside(12),3,3’-diacetyl-4,4’-dimethoxyl-2,2’,6,6’-tetrahydroxyl-diphenylmethane(13),2,4-dihydroxyl-6-methoxy acetophenone(13),2,4-dihydroxyl-6-methoxyl-3-methylic acetophenone (15), quercetin-3-O-β-glucopyranoside(16), obtusifoliol(17) and β-sitosterol(18).Thirteen compands were isolated from Piper Jlaviflorum C.DC. and four were idtentified. Piperine(1) was rich in Piper flaviflorum C.DC.We selected euphopilososide A(1), euphopilososide B (2), euphopilosolide (3) jolkinolide A(4), jolkinolide B(5),17-hydroxyjolkinolide A(6), phorbol(9), langduin A(10),13-hydroxy-ent-abiet-8(14)-en-7-one(11), which were evaluated for their cytotoxicities against five cancer cell lines and the result indicated that only compand (5) exhibited some activities.