Preparation Of The Molecular Imprinted Film For Ciprofloxacin And Study On The Electrochemical Detection

Electrogenerated Chemiluminescence (ECL), is directly or indirectly initiated by the electrochemical and chemical luminescence, has attracted many extensive studies in analytical applications. The ruthenium tris(bipyridine)(Ru(bpy)32+) and its derivatives are the most extensively studied complexes in ECL. However, the selectivity is poor. in analysis process. Molecularly imprinting technique has become very important in recent years. Generally, the imprinting process consists of rearrangement of the target molecules with functional monomers prior to initiation of polymerization, molecular imprinted polymer specific template molecule on the target identification and capture of template molecular will be selectively separated from the complex systems. The main results were as follows:(1) A self-designed ECL analysis system was fabricated. The ECL analysis is a new method with high sensitivity and has wide applications in many special fields. But there is a lack of high quality special instrument for research or practice of this method in our laboratory. It has been designed as a computer embedded one with facility of operation and powerful datum processing function. At the same time, a novel electrochemiluminescent cell based on indium tin oxide(ITO) and teflon material was designed and fabricated. It had solved the problems and provided a useful hardware for further study as well. The cell has many advantages and innovations.(2) An ECL inhibition method was developed for quantitative determination of ciprofloxacin(CPX). It was found that CPX strongly inhibited the ECL signal of Ru(bpy)32+-DBAE system. Based on ECL signal changes, a simple and ultrasensitive detection method for CPX was established. Under the optimized conditions, the quenched ECL intensity versus the logarithm of the concentration of CPX is in good linear relationship over a concentration range from5.0×10-7to1.0×10-9mol/L, with a detection limit of4.6×10-10mol/L(S/N=3).(3) An electrochemical sensor for ciprofloxacin was prepared by electropolymerizing o-phenylenediamine (o-PD) and resorcinol on a ITO electrode in the presence of ciprofloxacin, followed by detection using a cyclic voltammetry (CV) method. The morphology of sensor was characterized by scanning electron microscopy (SEM). Under the optimized conditions, the performance of the sensor was studied by square wave voltammetry(SWV). The results demonstrated that the MIPs electrochemical sensor not only possessed specific adsorption and high recognition capacity toward CPX but also could be used to detect it in the concentration range between5.0×10-10～1.0×10-7mol/L, with a detection limit of2.1×10-10mol/L(S/N=3). The sensor can also be applied in the detection of ciprofloxacin in real sample with satisfied result.(4) Based on Ru(bpy)32+-Au nanoparticles decorated ITO electrode and molecularly imprinted polymer (MIP), we explored an novel molecularly imprinted Electrochemiluminescence sensor. It’s electrol and electrochemiluminescence ability were tested. This work may be an good attempt for building sensitive method, which combined both the advantages of MIP and ECL.