Effect Of One-Dimensional ZnO Micronmaterial Interface On BSA Conformation

Along with the closer relationship between micro/nanomaterials and humanbeing, the excellent physical and chemical properties of the micro/nanometermaterials are realized. And thus the micro/nanomaterials have a wide range ofapplications in biomedicine. The introduction of the micro/nanomaterials may haveeffect on structure and activity of the biomolecules. It has become one of the keychallenges on how to further reveal their interface interaction mechanism at themolecule level. In the present dissertation, biocompatible ZnO micromaterial waschosen as a model material. The influence of their surface properties on theconformation of bovine serum albumin (BSA) was investigated after ZnO was treatedwith light illumination and functional groups. The details are as following：1.Preparation of one-dimensional ZnO micromaterial and conjugation withBSA：One dimensional ZnO micromaterials were preparated by hydrothermal methodand characterized by optical microscopy, scanning electron microscope and X-raydiffraction and fluorescence spectrometer. One dimensional ZnO micromaterials withdifferent surface properties were obtained through the treatment of ultravioletirradiation, dark storage in vacuum or different surfactants. The conjugation with BASwas performed by using the static adsorption or chemical coordination method. Theresult of EDS and Fourier infrared spectroscopy (FTIR) were confirmed the formationof the conjugates.2. Effect of one-dimensional ZnO micronmaterial interface on BSAconformation: FTIR analysis result to the amide I of BAS indicated that α-helices andβ-sheets of BSA are more sensitive to the surface property change of BSA than turnsand random coils, and the more hydroxyl groups exist on the surface of ZnOmicromaterials, the more influence it has on the secondary structure of BSA.