Optimization Of The Bitespiramycin Fermentation Technology

The impact of inorganic phosphorus on the synthesis of antibiotics is already familiar to people, Fermentation of antibiotics must be carried out under the premise of limiting inorganic phosphorus during the production process of many antibiotics,It means that the concentration of inorganic phosphorus must be controlled at a suboptimal level. If the fermentation process low concentrations inorganic phosphorus of the initial culture medium was inevitable depleted.it may have a negative impact of antibiotic fermentation.Inorganic ammoniumions salt is commenly used as nitrogen in the Actinomycetes culture.But the synthesis of many Macrolide antibiotic was relatively suppressed by high concentration of ammoniumions,such as Erythromycin,Lincomycind and Taylor star,This inhibition can be removed by adding ammoniumions trapping agent in the fermentation medium.As everyone knows that,Magnesium phosphate can be used as one of the ammoniumions trapping agents which effectively limit the concentration of ammoniumions in the medium. Due to its special chemical properties,Mg3(PO4)2can solwly supplement with soluble phosphorus into the fermentation liquid.This article researchs on the effect of Magnesium phosphate in Bitespiramycin fermenation process.Study the effect of Magnesium phosphate on fermentation components at different concentrations and different times when produce Bitespiramycin in composite medium.Adding0.1%,0.3%,0.4%,0.6%.0.9%,1%,1.5%of Magnesium phosphate in fermentation medium at0h,24h,36h.Feeding of Magnesium phosphate during fermentation was found to know the Isovaerylspiramy components of Bitespiramycin and the ratio of Isovaerylspiramy Ⅲ to Isovaerylspiramy Ⅱ.The result shows that:(1)Adding0.3%-0.4%of Magnesium phosphate can significantly improve the Bitespiramycin components at24h fermentation process.the titre (2058.719μ/ml) increased more than30%compare with the control group(1450.722μ/ml). Based on the data of fermentation process,such as residual sugar, total sugars,nitrogenions,the mechanism of Magnesium phosphate and the effect of sugar and nitrogenions on the fermentation process.(2)In order to verify the effect of titre on Bitespiramycin by using mass production of Magnesium phosphate,proceeding an amplification experiment on Bitespiramycin medium by using12L Fermentation tank,using high temperature and high pressure treatment and adding0.3%-0.4%Magnesium phosphate at fermentation24h as the same as the Shake-flask experiments.The result shows that the titre reaches a maximum value at fermentation84h compare with the experiment without adding Magnesium phosphate.