Preparation Of Perennial Vine Beans Cleavage Peptide And Study On Health Function

Perennial vine bean(PVB) is a new state invention patent varieties.lt have good comprehensive and characteristic, is a functional product of high nutritional value.The mature perennial vine beancontent of40%protein (Contain component proteins of18kinds of amino acids,especially8essential amino acids content high and reasonable proportion,belong to a complete protein),the oil content of15%or so.lt contains a lot of the full asking price of protein,18kinds of amino acid,the matching equilibrium,and essential amino acid content is rich,as much as50%or more.It is a kind of the higher quality protein resources than soybean protein’s value.Perennial vine beans cleavage peptide (PVBCP) adopt electromagnetic lysis,use of the proprietary equipment,its Patent number:CN200420059838.X.After experimental studies show that the best voltage of electromagnetic lysis is10.25KV, peptide content is3.2%; Extract the best technical parameters for solid-liquid ratio is1:3.5,soaking time is4min,the temperature of soaks raises is45℃.Get cracking peptide liquid brown transparent liquid,peptide content is6.1%.Testing to perennial vine beans cleavage peptide,results show that it with function of antioxidant、 Anti-fatigue and immune Significantly as follow:(1)Through the determination of resist fatigue and immune time from the survival of the mice,the test show that Perennial vine beans cleavage peptide can increase oxygen resistance in mice obviously.(2)Perennial vine beans cleavage peptide can influence the content of malondialdehyde (MDA) in the blood of mice,the results showed that mice in the blood lipid peroxide content was lower than those in the control group,it means that has the obvious antioxidant effect.(3)Perennial vine beans cleavage peptide results showed that the mice swimming time,climbing pole time,serum urea nitrogen,liver glycogen and muscle glycogen can delay fatigue.(4)Perennial vine beans cleavage peptide to blood lactic acid in mice influence of the results indicate that has a positive role in elimination of physical fatigue.(5)Perennial vine beans cleavage peptide not only improve the inhibitory amino acids (gamma-aminobutyric acid) content,but also improve the content of excitatory amino acids (glutamic acid).(6)Perennial vine beans cleavage peptide can adjust the metabolism and rapid recovery the physical strengthening,it provide a pharmacological basis for field research of Chinese medicine antibody fatigue.(7)Perennial vine beans cleavage peptide tested the growth of mice and establish the high cholesterol comparing models,the results show that it can significantly reduce the serum cholesterol,as well as increased SOD’s activity,Prevent lipid peroxidation.(8)Perennial vine beans cleavage peptide can significantly enhance the proliferation of B lymphocytes and T lymphocytes,Enhance murine macrophage phagocytosis of vitality and Generate a hemolysin antibodies,Significantly enhanced the immunity of cellular and Humoral.