Identification And Screening Of Strain Transforming Rare Ginseng Saponins And Optimization Of Transforming Conditions

Ginseng, the root of Panax ginseng C. A. Meyer, Araliaceae, is a well-known medicinal plant. It has been used as atraditional natural medicine in China, Japan and Korea, for thousands of years. Its chemical properties and pharmaceutical functions, such as anti-aging, anti-inflammation, anti-oxidation and so on, have been intensively studied throughout the world. Gingenosides are the main pharmacological active component of ginseng. Up to now, more than40ginsenosides have been isolated and characterized from ginseng roots, which including with the major ginsenosides Rb1, Rb2, Re, Rg1and Re that constituting more than90%of the total ginsenosides. The rare ginseng sapoins’pharmaceutical functions have been concerned by many people. And their get way also have been concerned by many people.The main purpose of this paper was to screen the strains that can carry out rare ginseng saponins transformation from TongHua forest soil and optimize the transforming conditions. The strains with the highest transformation activity were anticipated which was significant for the effective usage of ginseng and devoted to medical cause.Firstly, ginseng total saponins were separated from ginseng by using ethanol distillation and further glycol type ginseng saponins mixture by the column chromatography which including ginsengside Rb1、Rb2、Rc、Rd. The mixture was used as the substrate.Secondly,92strains were screened from the soil. The primary screening result showed that four strains can transform ginsenoside Rb1to ginsenoside Rd. The stain TH-10had the strongest transformation activity among them,The conditions of HPLC were studied for getting a more accurately qualitative and quantitative analysis result. The best condition of HPLC was attained as follows: chromatographic column:Ascentis Express C18(5cm×3.0mm,2.7μm), Mobile phase water(A)、acetonitrile(B), Column temperature:35℃, wavelength:203nm,1.0ml/min,5μl.Thirdly, the strain TH-10was identified belonging to Aspergillus, through the observation of the culture on plate, microscopic characteristics and molecular biology method.Finally, the study optimized the best conditions of inoculum age, temperature, pH and substrate concentration. The result showed that the highest conversion rate was73.77%with one factor a time method, when the inoculum age, temperature, pH and substrate concentration were48h,5,27℃and0.15mg/ml,respectively.