Isolation, Characterization And Bioaugmentation Of High Salinity Aniline-Degradation Bacterial Strains

The aniline commonly known as Ani Lin oil, it is colorless or light yellowtransparent oily liquid and it has a strong pungent odor. As an important chemical rawmaterials, aniline is mainly used in the following industries: national defense, dyes,rubber, pesticides, pharmaceutical. With the rapid development of chemical industry, thedemand of aniline is increasing, also the total amount released into the environment ismore and more. So that it can cause serious environmental pollutionAniline wastewater with high salt not only contains aniline and other organicpollutants, but also has the characteristics of high salt. Although the inorganic ions playan important role in the process of the microbial growth, but it will be toxic tomicroorganisms, if the salt concentration is too high. For the treatment of Anilinewastewater with high salt, the traditional physical and chemical methods can not degradeit efficiently. So microbial treatment technology with great potential is the better choice.That is, separate and filter out the strains, which have a higher degradation rate of anilineunder the high-salt environment. And apply them into practice. It can provide theoreticaland technical support for tne treatment of Aniline wastewater with high salt.Four high efficient degradation strains which utilize aniline as sole carbon sourceare isolated from activated sludge. And the activated sludge is from aeration tank of thenortheast general pharmaceutical factory. According to the Identification andDegradation Characteristics of the strains, the strain aa-2and strain cc-4are identified asMicrococcus sp.; the strain aa-8and strain bb-1are identified as Corynebacterium sp..The results of degradation characteristics show that the optimal temperature, pH value,inoculum size and shaker rotary speed of four strains are respectively30℃,7.0,10%and150r/min. The degradation rate is up to90%in48h when the aniline concentration is500mg·L-1. In addition, the tolerance to salt of strain aa-2is up to8%, strain aa-8, strainbb-1and strain cc-4is up to10%.High efficient degradation strains screened in laboratory were tested in SequencingBatch Reactor (SBR) to carry on the bioaugmentation. There is a good removal effect onaniline, TOC, MLSS, MLVSS and SV in the running system. Thus it can reduce theinhibitory effect of pollutants in the wastewater.