| Carbon nanomaterials have interesting chemical and physical properties,especial Cup-stacked-type Carbon Nanotubes (CSCNTs) and Graphene, andinvestigating the biological applications of new carbon nanomaterials is oftheoretical and practical significances. The purpose of this study is to investigate thepotential biological applications of the CSCNTs and Graphene, according to theirunique structures and properties. The contents of this thesis include:(1) magneticCSCNTs for protein separation;(2) APBA–functionalized graphene oxide forantibodies (IgG) cupture.Owing to their unique one-dimensional structures and desired magneticproperties, CSCNTs with magnetic nanoparticles trapped in their tips can serve astrain-like systems for protein separation. In this study, we functionalized themagnetic CSCNTs with high density of carboxyl groups by radical addition, and thenanchored3-aminophenylboronic acid (APBA) through amidation reaction to achieveoriented conjunction of antibodies (IgG). It was also demonstrated that the obtainedmagnetic CSCNTs–APBA–IgG conjugates could readily react with target antigensthrough specific antigen-antibody reaction and be used as new magnetic systems forprotein separation.Graphene is the most recently recognized member of the fullerene family.However, by the lack of large quantities of the material and its insolubility and greattendency to agglomerate in aqueous biological environment present obstacle towardsits biological applications. Differernt from graphene, graphene oxide (GO) has goodsolubility in aqueous biological environment. The reactive oxygen functional groupsof graphene oxide render it a good candidate for bioapplications through chemicalfunctionalizations. In this study, we functionalized the graphene oxide (GO) with3-aminophenylboronic acid (APBA) through amidation reaction to achieve orientedconjunction of antibodies (IgG). It was also demonstrated the obtained NGO–APBAare highly water-soluble and can be used for protein separation. |
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