Study Of Hazardous Organic Matters Based On Three-dimensional Fluorescence Coupled With Second-order Calibration

Hazardous organic matters are widely distributed in nature and hardly degradable, from which toxic bioaccumulation usually occurs. Development of an efficient method for detection and control of these hazardous organic matters is of great significance for the protection of human life and ecological environment. Generally, organic harmful substances possess flat or nearly flat ring structure, whose electronic and chemical bond length of the molecular system tend to be average. They can be analyzed by fluorescence spectroscopy with high sensitivity due to their special spectral characteristics. However, due to the existence of the overlap of the fluorescence spectra of various substances and interference, this method was restricted in the actual complex system.Chemometrics, especially three-dimensional data analysis method has the advantages of the overlapping spectra in the quantitative analysis of the presence of the interference. The nascence and development history of chemometrics are introduced concisely in the preamble of this article, and especially so is the research progress of multi-resolution and correction methods, as well as their application. Accordingly, three-dimensional fluorescence detection coupled with multi-re solution and correction methods was applied to the detection of harmful organic matters in the environment and food system, to achieve a rapid multi-component substances quantitative determination of the interests in the coexistence of the unknown interferences, which provides a new way for the efficient determination of harmful organisms in a complex system.Water pollution caused by phenols is harmful to not only the environment, but also the human and aquatic life. Therefore, it is of great significance to develop a rapid and effective determination method. Three-dimensional fluorescence combined with second-order correction method was applied to isochronal determine phenol, hydroquinone and catechol in the urban sewage. The established method requiring simple pretreatment meanwhile avoiding prior separation, is capable of rapid quantitative determine of analytes in the sewage even spectra interference occur.A more efficient determination of pollutants is imperative for improving soil quality when soil pollution issues have been increasingly concerned in recent years. Simultaneous quantitative analysis of three phenols (bisphenol, naphthol, and hydroquinone) was performed by three-dimensional fluorescence detection coupled with second-order calibration algorithms in the Chapter III of this article. Interested targets in the complex matrix soil were rapidly detected in the absence of pre-separation. The process is featured of high recovery and good reliability.Pesticide residues are mainly detected by chromatographic analysis method, which is inconvenient, inefficient and time-consuming. In order to establish a low-cost, simple and fast method for simultaneous quantitative analysis of many pesticides, Three-dimensional fluorescence detection coupled with second-order calibration algorithms were used for the detection of the carbaryl and isoprocarb residues in pear and sweet potato in chapter IV of this paper. By studying the pH value of the measured system and the sensitizer effect, the three-dimensional data was obtained under optimal conditions. The analytical result shows that the method is a simple, rapid, and effective method for quantitative analysis of the contents of the pear and sweet potato despite the spectral overlap interference of multiple analytes without complex pretreatment.