Study On Quorum Sensing Of Biofilm Treatment In Aquaculture Wastewater

The quality of aquaculture water decides the output and quality of the products. Ourcountry is facing the problems of the shortage of water resources and environmentalpollution. Using scientific and effective methods to clean the aquaculture waster waterand recycle it can decrease the damage of the waste water and demanded quantity ofwater resources. Quorum sensing system conducts a newly developing research fieldthat has great potential to enhance the treatment effect of aquaculture waste water andincrease the utilization rate and application of waste water.Biofilm wastewater treatment technology is an important part of the recirculatingaquaculture system, which has been widely used. Biofilm wastewater treatmenttechnologies can effectively reduce the ammonia nitrogen and organic matter contentand purify the quality of water. Its mechanism is the use of microbial metabolism inthe biofilm consumed in water of harmful substances. This research is based on thebiofilm aquaculture wastewater treatment technology combined with the theory ofquorum sensing. We conclude that quorum-sensing signal molecules (AHLs) have animpact of the effect of bioflim wastewater treatment in aquaculture based on thebioflim wastewater treatment as the research object by analysing the experimentaldata used EXCEL and SPSS software.1. Prompting the growth and adherence of microorganism. The biomass of theadherence shows that the groups of N-3-oxo-C8-HSL and C6-HSL obviouslyincrease. Specially, the biomass of the group added N-3-oxo-C8-HSL is about sixtimes than the control group.2. There are some impacts on the removal rates of ammonia nitrogen, nitrite andphosphorus.3. Using SPSS13.0software calculates the synthetical score of each group by the factor analysis. The score of alcoholic group is lower than the controlled groupfrom beginning to the end. The two groups added N-3-oxo-C8-HSL and C6-HSLhave high marks in the day9to18days so those can state that the environment ofthese two groups are better in the process of this study. AHLs(N-3-oxo-C8-HSLand C6-HSL) have the effects of improving and enhancing water quality. However,the scores of the two groups added N-3-oxo-C8-HSL and C6-HSL are lower thanthe controlled group because of the high density in bioflim or the hostileenvironmental conditions (The determination of biomass also confirmed this).4. Nitrobacteria are strains of long growth cycle that is generally five days tostationary phase. By adding different concentrations of C6-HSL andN-3-oxo-C8-HSL to the culture medium of the nitrifying bacteria and nitritebacteria, the stationary phase of bacteria in advance about three days, andexperiments show that high concentrations of C6-HSL and N-3-oxo-C8-the HSLdoes not play a significant role in promoting the growth of nitrifying bacteria andvery much in line with the quorum sensing research reports. The highconcentrations of AHLs enable bacteria to produce the appropriate physiologicalregulation of behavior and inhibit or turn off the quorum sensing system.5. Our team separated and purified the nitrobacteria and nitrite bacteria from thecirculation aquaculture system and detecting them with16s RNA method.However the result of thin layer chromatography cannot show the accurateinformation.The researches concerning quorum sensing in aquaculture wastewater treatment havemany aspects to be resolved and improved. In this study, it is only a preliminary testand determination of quorum sensing in aquaculture wastewater. We also need to be more comprehensive and more in-depth study and analysis of finding out the key ofbacteria quorum sensing in the biofilm aquaculture wastewater treatment. The finalaims is to improve the quality and output of farmed animals. Meanwhile, in the aspectof reducing the environmental pollution also has a huge development potential andpositive role.