Study On Preparation Of The Main Triterpene Glycosides From Pearsonothria Graeffei And Study On Losing Weight

Sea cucumber is a costful tonic sea food which contains multi-kinds of bioactivecomponents. It is well known that holothurians are of high nutrition and pharmicvalues. Triterpene glycosides, one of the most important bioactive compositions ofsea cucumbers, are proved to exhibit various biological activities, includinganti-tumor, antifungal, immunomodulatory, cytotoxic and hemolytic effects.There areabundant compounds which have subtle differences with structural similarity. Thesaponin ingredients of sea cucumber are different due to the differences ofclassification and growth environment of sea cucumber. Therefore multiplechromatographic techniques are essential for separating and purifying holothurianglycosides monomers. Acording to previous study of our laboratory, saponins of seacucumber could play a part in reducing hepatic lipids accumulation and in the fattyliver rats, as well as serum TG and TC concentration.it could ameliorate fatty liverand insulin resistance. However, saponins of sea cucumber include abundantcompounds with similar structure. Their structure activity relationship is not clear.It isessential to separate the main monomers. Mading a comparative research into theeffect of the main monomers on disordered glucose and lipid metabolism in db/dbmice could provide scientific data for the structure-activity relationship of seacucumber saponinsThis paper takes Pearsonothuria graeffei as raw material to prepare holothurianglycosides, separate and purify the main monomers, Holothurin A (HA) andEchinoside A (EA). Then this paper makes a comparative study on the effect of themain monomers on disordered glucose and lipid metabolism in C57BL/KsJ (db/db)mice. The main results of this research are as follows:1. The preparation of holothurian glycosides monomers. Holothurin A (HA) andEchinoside A (EA) were separated from Pearsonothuria graeffei.Holothurianglycosides are soluble in hydrous butanol or pentanol with the phenomenon of delamination. Saponins of sea cucumber were separated using ethanol extraction,macroporous resin, normal-phase silica gel chromatography with the purity of75%.The main two monomers were separated from saponins of sea cucumber goingthrough reverse phase silica gel. The purity of two monomers was upto87.5%and91.0%.2. The effect of the main monomers on disordered lipid metabolism in db/dbmice. This paper takes Holothurin A (HA) and Echinoside A (EA) as raw material tostudy the effect of the main monomers on disordered lipid metabolism in db/db mice.The results show that two monomers both could inhibit weight gain and reduce theweight of adipose tissue.They could reduce hepatic lipids accumulation in the fattyliver rats, as well as serum TC concentration. Holothurin A could reduce hepatic lipidsaccumulation by inhibiting synthesis of lipids.The function mechanism of reducinghepatic lipids accumulation of Echinoside A is not clear. Holothurin A is moresuperior in improving lipid metabolism than Echinoside A.3. The effect of the main monomers on disordered glucose metabolism in db/dbmice.the C57BL/KsJ (db/db) mouse is the animal model of spontaneous obesity withdiabetes mellitus which is similar to the Type2Diabetes of human. The results showthat two monomers both could reduce fasting plasma glucose and alleviate impairedglucose tolerance and insulin resistance. Echinoside A is more superior in improvingglucose metabolism than Holothurin A.This research made quantitative analysis of the separating of the main twomonomers that could provide reference for large-scale preparation of holothurianglycosides monomers.meanwhile, This paper takes C57BL/KsJ (db/db) mouse asanimal model for the first time and study on the effect of the main monomers ondisordered glucose and lipid metabolism in C57BL/KsJ (db/db) mice.it could providedata support for the structure-activity relationship of sea cucumber saponins.