| People are increasingly interested in Benzo(a)pyrene of polycyclic aromatic hydrocarbonsand others toxic substances, especially the food contamination caused by them. Benzo(a)pyreneare widely distrbuted, stable, carcinogenicity and the food is easily infected by them. Foodsecurity related to people’s physical and mental health, so it is very necessary to understandingthe pollution caused by benzo(a)pyrene. Because most of the food such as fruits and vegetables,require real-time field-testing. However, due to the complexity of the existing detection methods,quality inspection departments failed to real synchronization make this work. Therefore, thereneeds to establish a rapid and simple detection method. Surface-enhanced Raman spectroscopywith narrow emission band spectrum, high stability, capable of multi-component analysis,instrument, simple operation, high sensitivity in the detection of trace substances, and can bedetected in aqueous solutions and humid atmosphere, easily on the scene in real time for rapiddetection.This dissertation is dedicated to the preparation of a new simple, high sensitivity,surface-enhanced Raman substrate to detection using benzo(a)pyrene and other toxic andhazardous substances as a probe molecule. Using of freshly prepared substrate to implementthem at low concentrations to its qualitative and semi-quantitative detection. This dissertationincluds five parts as following:(1) literature review, Elaborate on the sources, toxicity, and the way of contaminated ofbenzo(a)pyrene Department polycyclic aromatic hydrocarbons; Introduceding the commonlyused analytical detection methods of benzo(a)pyrene department of polycyclic aromatichydrocarbons and the principles of surface-enhanced Raman; focusing on the substrateprocessing method for surface enhanced Raman and the substrate processing method used todetection of benzo(a)pyrene; Finally, proposing this dissertation research ideas.(2) Synthetizing zirconium phenylphozphonate and Au particles of oil phase. Themorphology, composition and nature of it is characterzated by scanning electron microscopy,XRD, IR, contact angle, polarization curves, the contact angle, as well as transmission and othersynthetic materials Characterization. Preparation of the zirconia surface enhanced Ramansubstrate by zirconium phenylphozphonate and achieving the detection of benzo(a)pyrene withthe concentration of10-6mol/L, duing to the enhancement of Au nanoparticles and π-π stacking interactions between zirconium phenylphozphonate and benzo(a)pyrene.(3) Synthetizing zirconium phenylphozphonate package of gold core-shell nanoparticles by alarge number of experiments to optimize the experimental conditions, Which were characterizedby XRD, transmission, Raman, and other means; prepared surface-enhanced Raman substratewith dodecyl mercaptan and thioglycolic acid as the coupling agent under the conditions, bothrealizing the detection of the Benzo(a)pyrene with the concentration of10-8mol/L by using ofthe pre-concentration effect from thiols to produce a hydrophobic environment, π-π stackinginteractions between zirconium phenylphozphonate and benzo(a)pyrene and the formation ofzirconium phenylphozphonate package of gold core-shell nanoparticles, which cut off the corewith the outside world, the electromagnetic field of core nanoparticles SERS activity, whateverit is a single-layer or multi-layer conditions.(4) preparation of surface-enhanced Raman Scattering substract by liquid/liquid and gas/liquid interface assembly of gold nanoparticles film. its properties were characterized byscanning electron microscopy, contact angle, XRD and so on; and using of this substrate forsemi-quantitative detection of benzo(a)pyrene and melamine in low concentrations at10-8mol/L, duing to the SERS effect from the dense, uniform, single-layer Au nanoparticles.(5) explored preparation of surface-enhanced Raman Scattering substract by the use ofanodic aluminum oxide template; optimized the conditions under constant current prepared by alarge number of experiments and scanning electron microscopy characterization to prepareregular, uniform anodic aluminum oxide template. |
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