| Food traceability techonology is an effective measure of the food chain risk management. Fattyacid composition and content of organisms closely related to the geographical environment of its growth,which provides the possibility of food traceability research and application. Fatty acid fingerprintanalysis of food traceability mainly focused on the plant-derived food such as olive oil, coffee. It’s notclear aboat the difference of fatty acid fingerprint information of animal-derived food tissues fromdifferent geographical sources, and the impact subject to region, feed, season and other factors is alsounclear.Beef is of nutritional quality and high value, which is one of the main meat of the people. But bythe effect of “mad cow disease”,“foot and mouth disease” of zoonoses, beef quality and safety issuesare on concern, beef provenance system is urgent to be established and improved. In this study, thefeasibility of the fatty acid fingerprint analysis used for the origin tracing of the beef is systematicalyanalyzed, as well as the fatty acid indicators effected by region, feed, and seasonal factors. The studyaims at establishing beef faty acid fingerprint tracing indicator system and discriminant model, andsupplying technology supports for the research and application of beef tracing.Firstly, this beef samples were collected randomly from the representative of China’s four majorbeef producing areas of Jilin, Ningxia, Guizhou, Hebei. According to the analysis of variance, multiplecomparisons and discriminant analysis, the fatty acid composition and content characteristics of the beefsamples were compared among four major regions, the indicators of beef origin discrimination wereanalyzed, the feasibility of the fatty acid fingerprint analysis for beef tracing were preliminary analyzed,the potential traceability index of fatty acid were preliminary choosed. And then according to controlingthe feed of cattle model test in Changchun city, Jilin province, the influence on the fatty acidcomposition and content in bovine muscle tissue by changing feed were studied; According to the cattlemigration model test of Taipusiqi in the three regions of Inner Mongolia, Yangling city, Shaanxiprovince and Nanyang city, Henan province, the effect of region, season and their interactions on thecomposition of the fatty acid content of bovine muscle tissue were analyzed. Finally, the initialestablished fatty acid index system and the discriminant model of beef traceability were verified andvalidated, the effective fatty acid index system was screened, and the discriminant model wasestablished. According to the analysis,the following conclusions were obtained:(1)The content and composition characteristics of fatty acid in beef samples from Jilin, Guizhou,Ningxia and Hebei are significantly different. The content of SFA are significantly higher in Jilin andHebei than that in Ningxia and Guizhou; the content of C16:1and C18:1in Ningxia are significantlyhigher than other areas; Guizhou and hebei obtain the extremely higher content of a-C18:3, C20:5andPUFA-n3than in Jilin and Ningxia. According to discriminant analysis, five potential indicators of a-C18:3, C14:0, C17:0, SFA and MUFA used for beef are preliminary screened. The overall classificationrate of four regions by the five indicators is82.0%. (2)In controling the feed of cattle model test in Changchun city, Jilin province, the content of SFAand C16:1are significantly different among different feeding groups，the other fatty acids a-C18:3,C14:0, C16:0, C17:0, C18:0, C18:2, C20:5, MUFA and n-3PUFA show no difference.(3)The beef samples from TP, YL and NY contain various content and composition characteristicsof fatty acid. There are significant difference of fatty acids C14:0, C16:0, SFA, C16:1, C18:1, MUFA,C17:0, C18:0, a-C18:3, C20:5and PUFA-n3. The contents of C15:0, C17:0, a-C18:3and C18:1differsignificantly among different breeding periods in TP; The fatty acid content show no significantdifference among different raising periods in YL. By the general linear model analysis, the sevenreference index of C18:1, C20:5, n-3PUFA, a-C18:3, C14:0, C17:0and MUFA which can be used tobeef origin traceability are filtered, and the samples of each feeding period are well distinguished, thegeographical characteristics are distinct.(4)a-C18:3, C14:0, C17:0and MUFA are relatively stable index system for beef provenance, theoverall discriminant rate of four beef-producing regions is83.6%, which shows good judging effect.(5)Fatty acid fingerprinting technology is a potencial technology of beef origin tracing. |
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