Preparation Of Novel Immobilization Materials And Their Application In Construction Of Electrochemical Biosensor

Immobilization material of biosensors based on redox proteins hasattracted considerable recent attention since it plays an important roal inprotection of proteins or enzymes and the construction of third-generationbiosensors. Immobilization material is the key factor of promoting thebiosensor performance. It makes plenty of researchers work on exploring thesuitable material for promising biosensor.In order to protect proteins under extreme conditions, the paper designand synthesis (ZrPNS/Mb-DNA)2films to fabricate biosensor. Target toimprove the effective collisions of the reactant molecules and protein, thepaper synthesized three-dimensional hierarchical porous graphitic carbon(3DC) to fabricate biosensor. More details are given as follows:(1) In this work, using ZrPNS, DNA and Mb as a component, we build abiosensor suitable for extreme conditions through layer-by-layer assembly. Inthe resulting films, the rigid2D nanosheets form the confinement space, makethe simple mix of DNA and Mb act like the firm complex,so that,it makes theimmobilized protein maintain their secondary structure well, and still showedgood catalytic activity under extreme conditions. In addition, (ZrPNS/Mb-DNA)2films also has a high protein loading, flexible proportionof component, and the advantages of simple preparation methods(2) After carbonation and etching, we prepare a three-dimensionalhierarchical porous carbon material, which was shown to be a promisingmaterial for Mb immobilization. Prepared H2O2biosensors excellent catalyticactivity is attributed to the three-dimensional hierarchical porous structure ofthe carbon material, the holes are more suitable for the immobilization ofbiological molecules, and macroporous inplanted holes act like a confinementspace, making the gather of the reactant molecules, which will greatly increasethe effective collision of protein and reactant molecules.