Study On Degenotoxicity Of Lactobacillus Salivarius From Macrobian

Lactobacillus salivarius FDB86was isolated from the fecal samples of macrobian of Bama, the "Fifth Longevity Area in the World".This paper is mainly focused on the potential for degenotoxic activity of this strain against4-Nitroquinoline1-oxide (4NQO) and the corresponding mechanism.Also Whether the ability of Lactobacillus salivarius FDB86to degenotoxic is strain specific is studied.In order to study the relationship of the structure and function of the active substances involved in the metabolism of4NQO Lactobacillus salivarius FDB86was coincubated with4NQO, and the active substances involved in the metabolism of4NQO was produced in quantity. This work mainly focused on the following aspects:1.Sos Chromotest is applied as the method to determine the ability of Lactobacillus salivarius FDB86to degenotoxic against4NQO.First E.coli PQ37-----the Indicator of Sos Chromotest was tested.It comes out that E.coli PQ37has the ability to produce β-Galactosidase and alkaline phosphatase.So it’s fit for use in chromotest.Then4NQO of different concentration was prepared and ananylised by HPLC. After linear regression analysis of the HPLC chromogram,we come to:y=30893x-195443, R2=0.9845.The degenotoxic ability against4NQO of Lactobacillus salivarius FDB86was separately detected by the classic Sos chromotest and HPLC.The corresponding results come out to be94.19%and82.92above.It indicates that Lactobacillus salivarius FDB86is a strain of strong ability to degenotoxic against4NQO.2.The effects of different concentrations of Lactobacillus salivarius FDB86on the process of Lactobacillus salivarius FDB86to degenotoxic against4NQO was studied by HPLC. It was found that low concentration of Lactobacillus salivarius FDB86converted4NQO into4HAQO,a more toxic intermediate reduction product.Then4HAQO change into4AQ, an end product of no toxicity,and little4AQ0,an end product of less toxicity in company.But high concentration of Lactobacillus salivarius FDB86,given enough dose or longer reaction time,can convert4NQO thoroughly into4AQ.That is to say Lactobacillus salivarius FDB86have the ability to completely suppress the genotoxicity of4NQ0.3.The ability of other35strains to degenotoxic against4NQO was tested by Sos chromotest and in the mean time by HPLC.Their HPLC chromogram were studied and ananylised,and their degenotoxic ability against4NQO were compared.lt is found that the strains which have high degenotoxicity ratio are one L.Planterum, five Bifidobacterium, L.casei Shirota, three Bacillus,two Saccharomyces cerevisiae. There is inconspicuous difference between the ability to degenotoxic against4NQ0of strains of one genus,but they are in diversity.Only Lactobacillus salivarius strains can produce P1after coincubation with4NQO.These indicate that the inhibitory ability of probiotics was strain specific.And the ability probiotics have to convert4NQO into4HAQO determines whether probiotics have high or low ability to degenotoxic against4NQO.4.Large quantity of Lactobacillus salivarius FDB86was produced by fermenter.The active substances involved in the metabolism of4NQO was produced in quantity by coincubate Lactobacillus salivarius FDB86with4NQO,then by micro filtration and Ultra Filtration.The product was condensed by vacuum freeze drying.