Study On The Interaction Of Gold Nanoparticles And Boric Acid Compound With Dye By Spectroscopy

Chapter1:This chapter provided the literature review, It reviewed the principle of fluorescence resonance energy transfer, the theoretical calculations, the common energy transfer acceptor-receptor and the outlook of the fluorescence resonance energy transfer. It provided the research background and the innovation.Chapter2:Synthesis of bovine serum albumin and human serum albumin protected water-soluble gold nanoparticles. Both nanoparticles are stable and difficult to gather. Their properties were characterizated by fluorescence spectrum and UV-visible absorption. The binding properties of fluorescein sodium with the two gold nanoparticles were investigated by fluorescence and UV-Vis spectroscopy and calculate the relevant parameters.Chapter3:The binding properties of P-(Diphenyeam ino) phenyl Boronic Acid (DPBA) with fluorescein sodium were investigated by fluorescence and UV-Vis spectroscopy.Apparent binding constants and corresponding thermodynamic parameters at different temperatures were calculated by Stern-volmer equation and thermodynamic formula, respectively. Results indicated that the quenching is static quenching and the electrostatic attraction could be in dominant position in the formation of the complex.Chapter4:The binding properties of P-(Diphenyeam ino) phenyl Boronic Acid (DPB A) with neutral red were investigated by fluorescence and UV-Vis spectroscopy.Apparent binding constants and corresponding thermodynamic parameters at different temperatures were calculated by Stern-volmer equation and thermodynamic formula, respectively. Results indicated the distance of donor-acceptor is2.439and the efficency of energy transfer is0.254. Moreover, we concluded that the quenching is static quenching and the hydrophobic interaction could be in dominant position in the formation of the complex.