Bion-modification And Applications In Cell Immobilization Of Gold Nanoparticles

With the increasing need of energy, there is a focus on attention problems in energy resources. However, there should be a great need to exploit new clean and environmentally friendly energy due to energy crisis brought by the limited resources.Photosynthetic cells, with their unique properties, can realize the solar energy storage and transformation. As one of the most extensively approaches applied in techniques, cell immobilization can be defined as a technique which provides living micro-environment that cell can survive in stable and controllable condition. Thus, cell immobilization has become a key technique in the utilization of photosynthetic cells. Au nanoparticles with advantages of high catalytic activity, good biocompatibility, stability, selectivity and easy to modify are beneficial for the construction of biocompatible interface, the modification of cell surface and the functionalization of cell encapsulation. Moreover, sol-gel immobilization technology based on silica is able to provide cyanobacteria hydrophilic and biocompatible living environment.This article discusses the cellular property, morphology and cell culture approaches by choosing Synechococcus7942. Pore structured biocompatible nanosized gold biohybrid layer was fabricated by the combination between nanotechnology and sol-gel encapsulation technology. This technique realized a double-layer encapsulation in order to provide a stable living environment for improvement of life span and photosynthetic efficiency.The first part of this article is the introduction which introduced the definition and properties of Au nanoparticles, followed by preparation method and applications. Subsequently, self-assembling of Au surface and biocompatible surface were also proved by Field Emission Scanning Electron Microscopy and Transmission Electron Microscopy which provided evidences that the nanosized gold biohybrid surface is biocompatible. In the end, the modified Au surface incorporated with sol-gel encapsulation technology, which constructed pore-structured double-layer encapsulation system was also investigated by controlling the volume ratio and proved by Confocal Laser Microscope, Oxygen Lab and Ultraviolet Spectrophotometer. The result indicated that encapsulated cyanobacteria can survive more than60days.