Metal Sulfur Protenin Genetic Engineering Fungus To Henvy Mental Waste Water Processins Research Application

Various countries are facing the varying degree water body heavy metal pollution, therefore current various countries widely carried out the the research about the heavy metal pollution. Uses the biological law to govern the heavy metal pollution is highly effective, the economical way, has avoided questions and so on two pollution which the conventional routes solve with difficulty, meets the present advocate sustainable development requirement.The research indicated that the metal sulfur protein (Metallothionein, is called MT) is a kind widely exists in the biosphere, including the cysteine, the low molecular weight, can richly by the metallic ion and other element induction metal conjugated protein, may through its massive cysteine residue chelated heavy metal, eliminate or reduce the water body heavy metal pollution.This topic was completes in predecessor’s foundation take has obtained from the jujube tree growth early time bearing branch cDNA small scale sequence including MT as a-2metal sulfur protein gene reorganization material particle pSPORT1template, increased through the PCR method reads frame’s ZjMT gene including the complete opening, the success constructs the nucleus fusion expression vector and, and transformed the backwoods coli to express strain BL21(DE3), after constructing the jujube metal sulfur protein nucleus to express the system. Inspected this metal sulfur protein genetic engineering fungus temperature and the PH tolerance with emphasis, meanwhile artificial has established static and the dynamic loop two kind of experimental model, inspected the genetic engineering fungus under the different response parameter to realistic simulation waste water heavy metal ion Cr2+, Ni2+, the Cd2+elimination effect, and analyzed has created the different result the primary cause. In addition, but also observes this metal sulfur protein genetic engineering fungus through the electron microscope under to adhere to stick cohere the condition under the padding, to inquire into initially it to simulates in the industrial waste the heavy metal ion elimination mechanism, the present paper divides into three parts:The first part:Genetic engineering fungus tolerant research.The second part:Genetic engineering fungus to heavy metal concentration.The third part:Circulation, dynamic system simulation genetic engineering fungus to heavy metal concentration. This experiment mainly in predecessor’s foundation, after the success construction and can express the jujube metal sulfur protein highly effective the project strain, simulates the different experimental condition, has studied the project fungus to the different metallic ion elimination rate research experiment, has laid the foundation for the following metal sulfur protein genetic engineering fungus’s in actual environment government applied research.