| In this paper, the effects of substrate concentration, bioaugmentation technology and surfactant on biohydrogen production was studied. Cow dung compost (or biogas slurry) was used as microflora and acid hydrolysis stalks and sucrose were used as substrates. The main contents are as follows:1. The hydrogen yield decreased with the increase of substrate concentration while the concentration was above20g/l.The hydrogen yield and hydrogen production rate fell from101.2ml/g and1.81ml·g-1·h-1to59.0ml/g and0.42ml·g-1·h-1while the stalk concentration rose from20g/l to90g/l.2. The hydrogen yield could be significantly improved by the strains synergistic effect. When the two pure strains named b and F which were separated by the laboratory were co-cultured at the ratio of1:1, the hydrogen yield was228.1ml/g sucrose, increased52%and40%compared to the control group of b and F, respectively.3. The effect of bioaugmentation on hydrogen production when using biogas slurry as microflora was studied by adding pure strain C41which was separated by the laboratory. The hydrogen yield was138.6ml/g corn-stalk,35%more than the control group of102.9ml/g corn-stalk, when the inoculum of C41was21%. When using sucrose as substrate, the maximum hydrogen yield was300.7ml/g sucrose,32%more than the control group, when the inoculum of C41was16%.4. To some extent, the hydrogen yield could be improved by adding surfactant, such as CTAB (short for cetyl trimethyl ammonium bromide), to the fermentative hydrogen production system. The hydrogen yield of corn stalk rose from114.2ml/g without CTAB to138.8ml/g with CTAB addition amount of30mg/1. When using sucrose as substrate, the hydrogen yield rose from the control group of220.7ml/g to283.6ml/g with CTAB dosage30mg/l. The verification experiment showed that in the dark fermentative hydrogen production system using sucrose as substrate and biogas slurry as microflora, the hydrogen yield could reach349.1ml/g sucrose when21%C41and30mg/l CTAB were added. |
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