| As in recent years, China’s economic development and improve theinternational status of the world trade in food has made significant progress,the current standard of living makes it the demand for a variety of meatproducts has greatly growth, as well as animal husbandry and aquaculture inChina has also been a rapid development. However, due to the vast majority offarmers lack of scientific knowledge and legal, health and other aspects ofcommon sense, coupled with huge economic interests driven farminghousehold excessive use of drugs or abuse of the illegal variety of forbiddendrugs in recent years from melamine to clenbuterol, a variety of harmfulantibiotics, additives and growth-promoting agent residues are not onlyharmful to the health of consumers, such as carcinogenic, mutagenic, toxiceffects on the human body, caused by the increase in bacterial resistance toantibiotics, but at the same time affect the import and export trade of livestockproducts.At present, food and drug residue detection methods includechromatography, immunoassay and microbiological assay. Thechromatography advantage of precise determination, but the instrument isexpensive, pre-treatment is more complicated and require professionals tooperate, and can not be achieved to a large number of rapid detection,microbiological method is simple, but only qualitative analysis, not quantitativeanalysis, tend to be higher than Zuigao Jian measurement sensitivity is nothigh.Our study ELISA to overcome the shortcomings of the above methods,both qualitative analysis of large quantities of precise quantitative analysis andpre-treatment is simple, rapid detection, there are broad prospects fordevelopmentELISA method, many researchers have synthesized a variety ofimmunogens, antibodies after immunization, but this area of the majority of biological experts, most of the transformation of various drugs only with asimple chemical method to connect, practicesrough to deal with simplyconcentrated dry, there is no reasonable deal with the synthesis, purification,and the related characterization, often just a simple analysis of whether thereis a spacer connected, so that subsequent antigen and antibody effects bothgood and bad is difficult to stable. This paper, I use several ways toractopamine, diethylstilbestrol, metronidazole several drugs, the total synthesisof a precise and effective purification. Connexins were some improvements,and the results were related to determination and analysis. Connection withBSA immunized New Zealand white rabbits ractopamine the diethylstilbestrolpolyclonal antibody. Then the titer of antibody and antigen using the ELISAmethod, sensitivity, and so on.In this paper, divided into two parts1） Ractopamine, diethylstilbestrol, metronidazole hapten with a varietyof methods for total synthesis and antigen preparation.In this paper, intoxicated by the succinic acid, glutaric acid anhydride,4-bromide, ethyl butyrate, iodoacetic acid and drug connections,synthesis of multiple hapten, and ractopamine multiple active sites ofreaction.2) Ractopamine diethylstilbestrol metronidazole antibody preparation,and establishment of ELISA detection method.Original synthesis of immune polyclonal antibody preparation of thecorresponding selection of the best of them were isolated and purified afterthe establishment of the ELISA method for detection of antigen synthesismethods, comparison of various methods of synthesis results and thedifferent active groups connection effect. |
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