Preparation Of An Anti-cardiovascular And Cerebrovascular Diseases, Drugs And Lipid-lowering Function

a-Linolenic acid (a-Linolenic Acid) for the cis-9,12,15-18carbon triple acid, as ω-3series polyunsaturated fatty acids have significant pharmacological effects and nutritional value, in the medical profession and nutrition academic concern. But the human body can not produce a-linolenic acid, only from exogenous food intake, in vivo and by desaturase enzymes of carbon chain extension of the catalytic synthesis of polyunsaturated fatty acids EPA (eicosapentaenoic acid) and DHA (docosahexaenoic acid). EPA is the body, such as leukotriene prostaglandin PGI3, TXA3and five Department of leukotriene precursors; DHA is the brain, retina and other nervous system components of membrane phospholipids. These two substances in the blood fat, lowering blood pressure, anti-thrombotic, anti-cancer, anti-allergy, etc. can play an important role. With the rise of health food products, a-linolenic acid has become an important health food base.The topics to linseed oil has a biological activity of a-linolenic acid (a-Linolenic Acid) Purification and lipid-lowering functional studies for the purpose of the lipase-catalyzed hydrolysis of linseed oil fatty acid mixture was investigated and the use of Urea legal separation of (3-cyclodextrin a-linolenic acid in linseed oil process conditions, and the isolated lipid-lowering function of a-linolenic acid were studied for the linseed oil in the functional development and utilization of a-linolenic acid to provide basis. Details are as follows:1. In this experiment, the water content of8.5%flaxseed, protein content19.3g/100g, oil was38.5%. Obtained by Soxhlet extraction of iodine as linseed oil178g/100g, saponification value of190mg/g, peroxide value4.8mmol/kg, acid value of0.18. Flaxseed and flaxseed oil the main indicators in line with national standards.2. Linseed oil is rich in a-linolenic acid. Amount of a-linolenic acid intake can not only improve the function of the nervous system, behavior and activities of learning and memory and can promote, but also regulate the immune system, reduce and prevent the occurrence and development of many diseases, and prolonging life, anti-aging effect. In order to maximize lipase hydrolysis rate of linseed oil, linseed oil in this experiment studied the effects of the hydrolysis rate of pH, reaction time, amount of emulsifier, enzyme concentration, oil/water ratio and other factors, Through the careful analysis of the experimental data, using mathematical software matlab, fitting to establish forecasting model, and then to test and verify, found in the temperature of33℃, pH=5.9,0.50of the oil/water,10%(oil) of the OP emulsifier, the amount of lipase13600u/g (oil), hydrolysis time29-hour process conditions, the hydrolysis rate can reach62.6%, for subsequent purification of urea inclusion and lay a good foundation.3. Using response surface analysis of Urea purified a-linolenic acid process conditions were optimized. Results:Through experiments the best conditions for the inclusion reaction mixture fatty acid:urea:ethanol(W/W/W)=1:3:7, inclusion temperature:-20℃, inclusion time:30hours, when a-linolenic acid content93.27%.4. In order to extract from the linseed oil of high purity a-linolenic acid, β-cyclodextrin on the legal separation of a-linolenic acid linseed oil in various factors has been studied. Through experimental analysis, the legal separation of β-cyclodextrin a-linolenic acid optimum conditions are:(Mixed fatty acid+ethanol):P-cyclodextrin=1:7,the inclusion temperature is59℃,the inclusion time is2.7hours, the freezing time is14.26hours. Under these conditions, a-linolenic acid content from3.84%to70.01%. The production process have high yield, low cost.It is simple and easy.It has good prospect for industrialization.5. The SPF level of50KM female mice are divided into the blank experiment group, model group and three experimental dose group. Study its α-linolenic acid lipid.Results: a-linolenic acid in linseed oil can reduce high blood cholesterol in mice serum cholesterol levels, triglycerides and LDL cholesterol levels, increased high-density lipoprotein cholesterol, atherogenic index of plasma can reduce, for small rats of hyperlipidemia and atherosclerosis significantly inhibited.