Preparation And Application Of Molecularly Imprinted Polymer For Nateglinide

As an emerging interdisciplinary method of molecular recognition, molecular imprinting technique (MIT) is developing quickly in recent years. Molecularly imprinted polymers (MIPs) can discriminate template molecule and analogs selectively. On account of their good sensitivity, stability and affinity, MIPs have been rapidly developed and widely used in environmental monitoring, clinical medicine, biotechnology, inartificial drugs, food industries and so on.Nateglinide [N-(trans-4-isopropylcyclohexycarbonyl)-D-phenylalanine] is a novel non-sulfonylurea oral antidiabetic agent for the treatment of type II diabetes mellitus. The methods for the determination of Nateglinide are HPLC, LC-MS and so on. In this paper, MIPs were synthesized using precipitation polymerization and their characteristics were valued. The results showed that the MIPs had high affinity and specificity to Nateglinide. Meanwhile, the polymers were applied to SPE and the preparation of electrochemical sensor.1. Preparation and characteristics of molecularly imprinted polymer for Nateglinide by precipitation polymerization. Molecularly imprinted polymers were synthesized using Nateglinide as template, AM as functional monomer, EDMA as cross linker and AIBN as initiator by precipitation polymerization. A number of issues that affect properties of the polymers were studied and polymer with a size of1μm was prepared.The maximum static adsorption capacities of the MIP and NIP for Nateglinide were0.83and0.22mg/g, respectively, which indicated that the MIP has a significantly higher binding capacity for Nateglinide than NIP. The competitive tests with similarly structural Repaglinide as competitor were performed to evaluate the recognition capability of MIP for template imprinting efficiency.2. The application of molecularly imprinted polymer for Nateglinide in solid phase extraction (SPE). Molecularly imprinted polymers synthesized under optimized conditions were used as the sorbent of SPE. By optimizing the loading, washing and eluting conditions, the MISPE was applied to the determination of Nateglinide in human plasma samples. The recoveries of Nateglinide in plasma samples were in the range of82.9%-86.6%.3. The preparation of electrochemical sensor for Nateglinide determination based on molecularly imprinted-carbon nanotubes. Based on the unique electronic properties and high adsorption capacity of carbon nanotubes as well as the specific recognition ability of MIPs, A novel electrochemical sensor constructed with imprinted polymer particles using Nateglinide as template and functional multi-wall carbon nanotubes (MWNTs) was prepared and evaluated. The sensor displayed a good linear range of2.0～160μg/mL under optimized pH and DPV conditions. The human plasma samples were determined with good results.