Upconversion Fluorescence Spectroscopy And Applications In Studies Of Biological Macromolecule Dynamics

Based on the principles of nonlinear sum frequency generation and pump-probe technique, frequency upconversion has become a femtosecond time-resolved detection technique since the femtosecond laser technology has been developed and popularized in recent years. Frequency upconversion fluorescence spectroscopy has played an important role in the studies of biological macromolecules’precise structures, functions as well as dynamics. Combined with the related optical and program design techniques and methods, a frequency upconversion experimental system has been designed and constructed professionally for the time-resolved fluorescence measurements in our efforts. With the help of this system, we have completed the time resolved fluorescence detection of several biological macromolecules and made the preliminary results.Fluorescence spectroscopy, time-resolved fluorescence detection and frequency upconversion technique are overviewed in this dissertation for a Master’s degree. Most importantly, the key design feature and solution to the self-constructed fluorescence upconversion system are demonstrated. The applications and achievements of the frequency upconversion in dynamic studies of tryptophan, proteins and DNAs are simply summarized. The experimental results in detecting the time-resolved fluorescence of dipeptide and quenched fluorescein by the self-constructed system are also presented. At last, summary and prospection of frequency upconversion technique and the detection system are discussed.