Biodiversity Of Airborne Microbial Flora During Dust Events In West Takalamakan Desert

In this research, culture-dependent and culture-independent (terminal restriction fragment length polymorphism, T-RFLP) methods have been used to studythe biodiversity and structure of airborne microorganism Flora before,during and after the dust events in Jiashi which locate in west Takalamakan desert.R2A medium plates was used to collect and investigate the airborne microorganism samples before, during and after dust weather in Jiashi, after isolationand culture we obtained238bacteria strains and25fungi strains, and used molecular identification method to classified these pure culture microbes. Bacteriawere divided into four phylum: Actinobacteria(47.06%), Bacteroidetes(1.26%),Firmicutes(21%),Proteobacteria(30.67%).Arthrobacter,Kocuria, Microbacterium,Stre-ptomyces,Bacillus,Planococcus,Paracoccus,Massilia were the dominant genera.Fungus were classified into8genera:Gibberella,Cladosporium,Alternaria,Davidiella,Penicillium,Peyronellaea,Trametes,Trichothecium,Cladosporium was thedominan species.Terminal restriction fragment length polymorphism, T-RFLP was the culture-independent mean which we used to learn uncultivable microorganism flora ofairborne microbes communities structure and diversity before, during and afterdust weather in Jiashi. It has been shown that uncultivable microorganism were divided into13phylum: Actinobacteria, Firmicutes, Bacteroidetes, Proteobacteria, Spirochaetes, Chlorobi, Deferribacteres,Aquificae,Thermomicrobia,Nitrospirae,Deinococcus-Thermus,Chlorobi,Acidobacteria.The uncultivable bacteria total abundance:Sample4(the sample collected in the non-dust day)>Sample3(the sample collected before dust weather)> Sample2(the sample collected after dust weather)> Sample1(the sample collected in dust events);Shannon Index:Sample1>Sample2>Sample3>Sample4.The dominant bacteria from non-dust day was Proteobacteria,in the dust day the dominant bacteria has been changed to be Bacteroidetes. The total abundance of airborne fungus significant difference:Sample1>Sample2>Sample4>Sample3; Shannon Index:Sample2>Sample1>Sample3>Sample4.The results of culture and culture-independent indicated that microorganismcan transfer with dust particles in the atmosphere that has an great influenceon microbial community structure and ecosystem in dust source areas and downwind area;Dust weather can substantially increase the concentration and typesof airborne microbes; Dust-borne microorganisms included many plant and animal pathogens; In the different times of dust weather, microbial communities were obviously different in the type and quantity of dust-borne microorganisms.