Panax Ginseng And Saccharomyces Cerevisiae Interactions And Screen Bacterial Idio-strain

Ginsenoside is zhe most important physiologically active ingredient in ginseng.It has been shown tohave anti-cancer, anti-fatigue, anti-Alzheimer’s and so on. It is a question how obtain the ginseng saponinslarge and rapidly. Microbiological expression is the most possible way to solve this problem, by theresearchers’ attention.It is a Ginsenoside biosynthetic pathway has been studied clear. Acetyl CoA biological into the IPP by the MVA pathway, And then IPP into squalene,an important precursors,squalene oxidized to2,3-oxidosqualeneby squalene epoxidase.At last oleanolic acid saponins and dammarane saponins oxide synthesized in differentsystem.Now,key enzyme in the biosynthetic pathway of ginsenoside has expression in Saccharomyces cerevisiae successfully. But Saponins biosynthesizing is a complex reaction, and requires a variety of enzyme-catalyzed. We can’t build a complete pathway by expression one or two enzyme what needed in zhe pathway in Saccharomyces cerevisiae. It is the biggest problem to face that ginsenosides cannot be expressed in microbial at this stage.In this study, extraction of successive transfer culture ginseng callus genomic Donavan use different methods transformed into saccharomyces cerevisiae:First,ginseng genome DNA transformation to saccharomyces cerevisiae cells.Second, the saccharomyces cerevisiae coating on the solid medium containing ginseng genome DNA and make its active absorption of exogenous gene to change their genetic structure.Third,Ginseng cell and saccharomyces cerevisiae cell fusion, create has ginseng and saccharomyces cerevisiae common traits of daughter cells.The original start and yeast expression systems expression in ginseng protein or recombinant peptide chain. Modify or extended the metabolic pathway of yeast synthetic terpenes existing. An ability to produce Ginsenoside or analogue need be discovery. Bacterial strain cans microbial conversion Ginsenoside Can is screened in it too.DAPI, a kind of fluorescent dye labeled that spike exogenous DNA in the experiment. Tests suggestedthat the yeast genome constituted after gene manipulation in three different methods, and under that ischanges in metabolic. Thread，transformat DNA into yeast directly, Yeast DNA and exogenous DNA istogether.DNA containing medium can’t be observed excited state, it is because high temperature cost DAPIactivity. Even so we found different colonial morphology. It suggests this method is effective. The final, inthe course of protoplast fusion and fusion cell growing, we seek for some cells specially. But these cellscannot grow and fission. The results suggested that the cell fusion can cause changes in the ginseng cells, butMost of this change is harmful.Thin layer chromatography (PLC) and high performance liquid chromatography (HPLC), we screenedone strains from a multitude of yeast strains, and named Y828-15-1. We detected two compound in this plantspecies has not yet been identified. Identification of the product in PLC, it position between ginseng saponinsRh2and Rh1on chromatogram.But unlike all ginseng saponins be detected early in HPLC detection, showthat the phase of matter in acetonitrile and water flow showed higher hydrophilicity.Although the twosubstances are not ginseng saponin, but may be some modification after biosynthesis, polysaccharidecontaining the base or excessive terpenoids.The metabolic changes in yeast is welcome, also is to have theresearch value. Both the nature and structure of the materials should be identified; genetic characteristics and genetic properties of stability still need further investigation.